The effects of in utero bisphenol A exposure on ovarian follicle numbers and steroidogenesis in the F1 and F2 generations of mice

Sharada Mahalingam, Laura Ther, Liying Gao, Wei Wang, Ayelet Ziv-Gal, Jodi A. Flaws

Research output: Contribution to journalArticle

Abstract

Bisphenol A (BPA) is a commonly used plasticizer. Previous studies show that in utero exposure to BPA affects reproductive outcomes in the F1–F3 generations of mice. However, its multigenerational effects on ovarian histology and steroidogenesis over the reproductive lifespan are unknown. Thus, we tested the hypothesis that BPA has multigenerational effects on follicle numbers and steroidogenesis. Mice were exposed in utero to vehicle control or BPA (0.5, 20, and 50 μg/kg/day). Ovaries were collected for histological and gene expression analyses and sera were collected for hormone assays. In utero BPA exposure decreased preantral follicle numbers, cytochrome P450 aromatase mRNA levels, and estradiol levels in the F1 generation, whereas it decreased testosterone levels and altered steroidogenic acute regulatory protein, cytochrome P450 cholesterol side-chain cleavage, 3β-hydroxysteroid dehydrogenase 1, and cytochrome P450 aromatase mRNA levels in the F2 generation. These data suggest that BPA has multigenerational effects on the ovary in mice.

Original languageEnglish (US)
Pages (from-to)150-157
Number of pages8
JournalReproductive Toxicology
Volume74
DOIs
StatePublished - Dec 2017

Keywords

  • Bisphenol A (BPA)
  • Diethylstilbestrol (DES)
  • In utero exposure
  • Multigenerational
  • Ovary
  • Sex steroid hormones
  • Steroidogenesis

ASJC Scopus subject areas

  • Toxicology

Fingerprint Dive into the research topics of 'The effects of in utero bisphenol A exposure on ovarian follicle numbers and steroidogenesis in the F1 and F2 generations of mice'. Together they form a unique fingerprint.

  • Cite this