TY - JOUR
T1 - The Effects of Genetic Relatedness on the Preterm Infant Gut Microbiota
AU - Lim, Shen Jean
AU - Aguilar-Lopez, Miriam
AU - Wetzel, Christine
AU - Dutra, Samia V.O.
AU - Bray, Vanessa
AU - Groer, Maureen W.
AU - Donovan, Sharon M.
AU - Ho, Thao
N1 - Funding Information:
Funding: This research was funded by NIH/NINR R21NRO13094 and RO1 NR015446 (MWG); NIH/NHLBI K23HL150300 (TH); and Carle-Illinois Collaborative Research Seed Proposal Program (TH, SMD). MAL was supported by a fellowship from the National Council of Science and Technology (CONACyT) from Mexico. SVOD received support from the Brazilian Federal Agency for Support and Evaluation of Graduate Education (CAPES) for graduate education.
PY - 2021/2
Y1 - 2021/2
N2 - The preterm infant gut microbiota is influenced by environmental, endogenous, maternal, and genetic factors. Although siblings share similar gut microbial composition, it is not known how genetic relatedness affects alpha diversity and specific taxa abundances in preterm infants. We analyzed the 16S rRNA gene content of stool samples, ≤ and >3 weeks postnatal age, and clinical data from preterm multiplets and singletons at two Neonatal Intensive Care Units (NICUs), Tampa General Hospital (TGH; FL, USA) and Carle Hospital (IL, USA). Weeks on bovine milk-based forti-fier (BMF) and weight gain velocity were significant predictors of alpha diversity. Alpha diversity between siblings were significantly correlated, particularly at ≤3 weeks postnatal age and in the TGH NICU, after controlling for clinical factors. Siblings shared higher gut microbial composition similarity compared to unrelated individuals. After residualizing against clinical covariates, 30 common operational taxonomic units were correlated between siblings across time points. These belonged to the bacterial classes Actinobacteria, Bacilli, Bacteroidia, Clostridia, Erysipelotrichia, and Negativicutes. Besides the influence of BMF and weight variables on the gut microbial diversity, our study identified gut microbial similarities between siblings that suggest genetic or shared maternal and environmental effects on the preterm infant gut microbiota.
AB - The preterm infant gut microbiota is influenced by environmental, endogenous, maternal, and genetic factors. Although siblings share similar gut microbial composition, it is not known how genetic relatedness affects alpha diversity and specific taxa abundances in preterm infants. We analyzed the 16S rRNA gene content of stool samples, ≤ and >3 weeks postnatal age, and clinical data from preterm multiplets and singletons at two Neonatal Intensive Care Units (NICUs), Tampa General Hospital (TGH; FL, USA) and Carle Hospital (IL, USA). Weeks on bovine milk-based forti-fier (BMF) and weight gain velocity were significant predictors of alpha diversity. Alpha diversity between siblings were significantly correlated, particularly at ≤3 weeks postnatal age and in the TGH NICU, after controlling for clinical factors. Siblings shared higher gut microbial composition similarity compared to unrelated individuals. After residualizing against clinical covariates, 30 common operational taxonomic units were correlated between siblings across time points. These belonged to the bacterial classes Actinobacteria, Bacilli, Bacteroidia, Clostridia, Erysipelotrichia, and Negativicutes. Besides the influence of BMF and weight variables on the gut microbial diversity, our study identified gut microbial similarities between siblings that suggest genetic or shared maternal and environmental effects on the preterm infant gut microbiota.
KW - Gut microbiota
KW - Human milk
KW - Preterm infant
KW - Triplets
KW - Twins
UR - http://www.scopus.com/inward/record.url?scp=85100023080&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85100023080&partnerID=8YFLogxK
U2 - 10.3390/microorganisms9020278
DO - 10.3390/microorganisms9020278
M3 - Article
C2 - 33572789
SN - 2076-2607
VL - 9
SP - 1
EP - 15
JO - Microorganisms
JF - Microorganisms
IS - 2
M1 - 278
ER -