The Effect of Urine Concentration and pH on the Growth of Escherichia Coli in Canine Urine In Vitro

L. A. Thornton, R. K. Burchell, S. E. Burton, N. Lopez-Villalobos, D. Pereira, I. MacEwan, C. Fang, A. C. Hatmodjo, M. A. Nelson, A. Grinberg, N. Velathanthiri, A. Gal

Research output: Contribution to journalArticle

Abstract

Background: Lower urinary tract infections are common in dogs, and Escherichia coli is the most common bacterial pathogen isolated. The literature has conflicting evidence regarding the inhibitory effects of urine concentration and pH on E. coli growth. Hypothesis/Objectives: To determine the effect of different pH and urine concentrations on E. coli growth in vitro. Animals: Voided urine samples from 10 apparently healthy spayed female dogs were used. Methods: A matrix of 9 urine specific gravity (USG; 1.010, 1.020, and 1.030) and pH (5.5, 7.0, and 8.5) combinations was prepared by diluting and titrating filtered voided urine samples. Three E. coli isolates were obtained from urine of female dogs with signs of lower urinary tract infection and cultured at different urine pH and USG combinations in wells of a microtiter plate. The number of E. coli colony-forming units (CFU) per mL of urine was calculated after aerobic incubation of the urine at 37°C for 18 hours, and statistically compared. Results: Significant differences were identified in the mean log CFU/mL among different combinations of pH and USG. The lowest log CFU/mL were observed in alkaline concentrated urine (pH 8.5 and USG 1.030). Conclusions and Clinical Importance: Escherichia coli in vitro growth was higher in neutral to acidic and diluted urine compared to alkaline and concentrated urine. The impact of non-alkalizing diluting diets on the incidence of E. coli lower urinary tract infections should be further explored.

Original languageEnglish (US)
Pages (from-to)752-756
Number of pages5
JournalJournal of veterinary internal medicine
Volume32
Issue number2
DOIs
StatePublished - Mar 1 2018
Externally publishedYes

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Canidae
urine
Urine
Escherichia coli
dogs
Growth
urinary tract diseases
Urinary Tract Infections
Stem Cells
Dogs
In Vitro Techniques
Specific Gravity
specific gravity
Diet
incidence
sampling
pathogens
Incidence

Keywords

  • Dog; E. coli
  • Lower urinary tract infection
  • Osmolality

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Thornton, L. A., Burchell, R. K., Burton, S. E., Lopez-Villalobos, N., Pereira, D., MacEwan, I., ... Gal, A. (2018). The Effect of Urine Concentration and pH on the Growth of Escherichia Coli in Canine Urine In Vitro. Journal of veterinary internal medicine, 32(2), 752-756. https://doi.org/10.1111/jvim.15045

The Effect of Urine Concentration and pH on the Growth of Escherichia Coli in Canine Urine In Vitro. / Thornton, L. A.; Burchell, R. K.; Burton, S. E.; Lopez-Villalobos, N.; Pereira, D.; MacEwan, I.; Fang, C.; Hatmodjo, A. C.; Nelson, M. A.; Grinberg, A.; Velathanthiri, N.; Gal, A.

In: Journal of veterinary internal medicine, Vol. 32, No. 2, 01.03.2018, p. 752-756.

Research output: Contribution to journalArticle

Thornton, LA, Burchell, RK, Burton, SE, Lopez-Villalobos, N, Pereira, D, MacEwan, I, Fang, C, Hatmodjo, AC, Nelson, MA, Grinberg, A, Velathanthiri, N & Gal, A 2018, 'The Effect of Urine Concentration and pH on the Growth of Escherichia Coli in Canine Urine In Vitro', Journal of veterinary internal medicine, vol. 32, no. 2, pp. 752-756. https://doi.org/10.1111/jvim.15045
Thornton LA, Burchell RK, Burton SE, Lopez-Villalobos N, Pereira D, MacEwan I et al. The Effect of Urine Concentration and pH on the Growth of Escherichia Coli in Canine Urine In Vitro. Journal of veterinary internal medicine. 2018 Mar 1;32(2):752-756. https://doi.org/10.1111/jvim.15045
Thornton, L. A. ; Burchell, R. K. ; Burton, S. E. ; Lopez-Villalobos, N. ; Pereira, D. ; MacEwan, I. ; Fang, C. ; Hatmodjo, A. C. ; Nelson, M. A. ; Grinberg, A. ; Velathanthiri, N. ; Gal, A. / The Effect of Urine Concentration and pH on the Growth of Escherichia Coli in Canine Urine In Vitro. In: Journal of veterinary internal medicine. 2018 ; Vol. 32, No. 2. pp. 752-756.
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AU - Fang, C.

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