The effect of glyceollin on proton leakage in Phytophthora megasperma f. sp. glycinea plasma membrane and red beet tonoplast vesicles

John L. Giannini, Jana S. Holt, Donald P. Briskin

Research output: Contribution to journalArticle

Abstract

Proton transport in sealed plasma membrane vesicles from Phytophthora megasperma f. sp. glycinea was inhibited by glyceollin. At 50 μM glyceollin no proton transport could be observed. Glyceollin at this concentration did not significantly affect the H+-ATPase responsible for proton pumping at the plasma membrane. Passive proton conductance increased significantly in the presence of glyceollin. These results suggest that glyceollin may inhibit fungal growth by diminishing the proton gradient necessary for solute uptake. Inhibition of the proton gradient appears to be due to increased proton conductance across the membrane in the presence of glyceollin and not inhibition of the H+-ATPase. Glyceollin also caused increased passive proton conductance in red beet (Beta vulgaris L.) tonoplast vesicles at levels where the ATPase was minimally inhibited. These data taken together suggest that a primary phytotoxic effect of glyceollin may be to cause proton or ion leakage across membranes.

Original languageEnglish (US)
Pages (from-to)39-45
Number of pages7
JournalPlant Science
Volume68
Issue number1
DOIs
StatePublished - 1990

Fingerprint

Phytophthora megasperma
Phytophthora
Beta vulgaris
tonoplast
beets
protons
Protons
plasma membrane
Cell Membrane
H-transporting ATP synthase
Proton-Translocating ATPases
glyceollin
Membranes
adenosinetriphosphatase
solutes
Adenosine Triphosphatases
microbial growth
Ions
ions

Keywords

  • Phytophthora
  • phytoalexin
  • proton conductance

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

Cite this

The effect of glyceollin on proton leakage in Phytophthora megasperma f. sp. glycinea plasma membrane and red beet tonoplast vesicles. / Giannini, John L.; Holt, Jana S.; Briskin, Donald P.

In: Plant Science, Vol. 68, No. 1, 1990, p. 39-45.

Research output: Contribution to journalArticle

Giannini, John L. ; Holt, Jana S. ; Briskin, Donald P. / The effect of glyceollin on proton leakage in Phytophthora megasperma f. sp. glycinea plasma membrane and red beet tonoplast vesicles. In: Plant Science. 1990 ; Vol. 68, No. 1. pp. 39-45.
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