The effect of chronic ammonia exposure on acute-phase proteins, immunoglobulin, and cytokines in laying hens

Y. N. Wu, F. F. Yan, J. Y. Hu, H. Chen, C. M. Tucker, A. R. Green, H. W. Cheng

Research output: Contribution to journalArticle

Abstract

Ammonia (NH3) is a potential health hazard to both humans and animals, causing local and systemic low-grade inflammation based on its levels and exposure durations. The objective of this study was to examine the effects of 45 wk of exposure to 30 ppm NH3 on the concentrations of acute-phase proteins, immunoglobulins, and cytokines in laying hens. At 18 wk of age, a group of Hy-Line W-36 hens was randomly assigned to 4-hen cages. These cages were evenly divided between 2 environmentally controlled chambers. At 25 wk of age, one chamber was maintained continuously with fresh air (NH3 < 5 ppm; control group) and the other one was injected with NH3 and controlled at 30 ppm (NH3 group) for 45 wk. At 70 wk of age, blood and spleen samples (n = 8 per treatment) were collected for analyses of immunological parameters. No significant differences were observed in plasma levels of albumin, complement components (C)-3 and C-4, immunoglobulin (Ig)M, IgA and IgG, interleukin (IL)-6, IL-10, or interferon gamma in the NH3 group compared to control group. Compared to control hens, NH3 exposed hens had higher plasma levels of α-1-acid glycoprotein (24%) and tumor necrosis factor alpha (54%) and higher mRNA expression of IL-1β (47%) and IL-6 (62.5%) in the spleen. These results indicated that hens may have the capability to adapt to chronic effects of moderate levels of NH3. Future studies should explore acute effects of NH3 at higher levels on hen health and welfare.

Original languageEnglish (US)
Pages (from-to)1524-1530
Number of pages7
JournalPoultry science
Volume96
Issue number6
DOIs
StatePublished - Jun 1 2017

Fingerprint

acute phase proteins
laying hens
immunoglobulins
long term effects
cytokines
ammonia
hens
interleukin-6
cages
spleen
immunoglobulin M
health hazards
interleukin-1
acute effects
interleukin-10
interferon-gamma
tumor necrosis factor-alpha
exposure duration
albumins
glycoproteins

Keywords

  • acute protein
  • ammonia
  • immunoglobulin
  • inflammatory cytokine
  • laying hen

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

The effect of chronic ammonia exposure on acute-phase proteins, immunoglobulin, and cytokines in laying hens. / Wu, Y. N.; Yan, F. F.; Hu, J. Y.; Chen, H.; Tucker, C. M.; Green, A. R.; Cheng, H. W.

In: Poultry science, Vol. 96, No. 6, 01.06.2017, p. 1524-1530.

Research output: Contribution to journalArticle

Wu, Y. N. ; Yan, F. F. ; Hu, J. Y. ; Chen, H. ; Tucker, C. M. ; Green, A. R. ; Cheng, H. W. / The effect of chronic ammonia exposure on acute-phase proteins, immunoglobulin, and cytokines in laying hens. In: Poultry science. 2017 ; Vol. 96, No. 6. pp. 1524-1530.
@article{54386bec36c247b8b822828d747d1e58,
title = "The effect of chronic ammonia exposure on acute-phase proteins, immunoglobulin, and cytokines in laying hens",
abstract = "Ammonia (NH3) is a potential health hazard to both humans and animals, causing local and systemic low-grade inflammation based on its levels and exposure durations. The objective of this study was to examine the effects of 45 wk of exposure to 30 ppm NH3 on the concentrations of acute-phase proteins, immunoglobulins, and cytokines in laying hens. At 18 wk of age, a group of Hy-Line W-36 hens was randomly assigned to 4-hen cages. These cages were evenly divided between 2 environmentally controlled chambers. At 25 wk of age, one chamber was maintained continuously with fresh air (NH3 < 5 ppm; control group) and the other one was injected with NH3 and controlled at 30 ppm (NH3 group) for 45 wk. At 70 wk of age, blood and spleen samples (n = 8 per treatment) were collected for analyses of immunological parameters. No significant differences were observed in plasma levels of albumin, complement components (C)-3 and C-4, immunoglobulin (Ig)M, IgA and IgG, interleukin (IL)-6, IL-10, or interferon gamma in the NH3 group compared to control group. Compared to control hens, NH3 exposed hens had higher plasma levels of α-1-acid glycoprotein (24{\%}) and tumor necrosis factor alpha (54{\%}) and higher mRNA expression of IL-1β (47{\%}) and IL-6 (62.5{\%}) in the spleen. These results indicated that hens may have the capability to adapt to chronic effects of moderate levels of NH3. Future studies should explore acute effects of NH3 at higher levels on hen health and welfare.",
keywords = "acute protein, ammonia, immunoglobulin, inflammatory cytokine, laying hen",
author = "Wu, {Y. N.} and Yan, {F. F.} and Hu, {J. Y.} and H. Chen and Tucker, {C. M.} and Green, {A. R.} and Cheng, {H. W.}",
year = "2017",
month = "6",
day = "1",
doi = "10.3382/ps/pew454",
language = "English (US)",
volume = "96",
pages = "1524--1530",
journal = "Poultry Science",
issn = "0032-5791",
publisher = "Poultry Science Association",
number = "6",

}

TY - JOUR

T1 - The effect of chronic ammonia exposure on acute-phase proteins, immunoglobulin, and cytokines in laying hens

AU - Wu, Y. N.

AU - Yan, F. F.

AU - Hu, J. Y.

AU - Chen, H.

AU - Tucker, C. M.

AU - Green, A. R.

AU - Cheng, H. W.

PY - 2017/6/1

Y1 - 2017/6/1

N2 - Ammonia (NH3) is a potential health hazard to both humans and animals, causing local and systemic low-grade inflammation based on its levels and exposure durations. The objective of this study was to examine the effects of 45 wk of exposure to 30 ppm NH3 on the concentrations of acute-phase proteins, immunoglobulins, and cytokines in laying hens. At 18 wk of age, a group of Hy-Line W-36 hens was randomly assigned to 4-hen cages. These cages were evenly divided between 2 environmentally controlled chambers. At 25 wk of age, one chamber was maintained continuously with fresh air (NH3 < 5 ppm; control group) and the other one was injected with NH3 and controlled at 30 ppm (NH3 group) for 45 wk. At 70 wk of age, blood and spleen samples (n = 8 per treatment) were collected for analyses of immunological parameters. No significant differences were observed in plasma levels of albumin, complement components (C)-3 and C-4, immunoglobulin (Ig)M, IgA and IgG, interleukin (IL)-6, IL-10, or interferon gamma in the NH3 group compared to control group. Compared to control hens, NH3 exposed hens had higher plasma levels of α-1-acid glycoprotein (24%) and tumor necrosis factor alpha (54%) and higher mRNA expression of IL-1β (47%) and IL-6 (62.5%) in the spleen. These results indicated that hens may have the capability to adapt to chronic effects of moderate levels of NH3. Future studies should explore acute effects of NH3 at higher levels on hen health and welfare.

AB - Ammonia (NH3) is a potential health hazard to both humans and animals, causing local and systemic low-grade inflammation based on its levels and exposure durations. The objective of this study was to examine the effects of 45 wk of exposure to 30 ppm NH3 on the concentrations of acute-phase proteins, immunoglobulins, and cytokines in laying hens. At 18 wk of age, a group of Hy-Line W-36 hens was randomly assigned to 4-hen cages. These cages were evenly divided between 2 environmentally controlled chambers. At 25 wk of age, one chamber was maintained continuously with fresh air (NH3 < 5 ppm; control group) and the other one was injected with NH3 and controlled at 30 ppm (NH3 group) for 45 wk. At 70 wk of age, blood and spleen samples (n = 8 per treatment) were collected for analyses of immunological parameters. No significant differences were observed in plasma levels of albumin, complement components (C)-3 and C-4, immunoglobulin (Ig)M, IgA and IgG, interleukin (IL)-6, IL-10, or interferon gamma in the NH3 group compared to control group. Compared to control hens, NH3 exposed hens had higher plasma levels of α-1-acid glycoprotein (24%) and tumor necrosis factor alpha (54%) and higher mRNA expression of IL-1β (47%) and IL-6 (62.5%) in the spleen. These results indicated that hens may have the capability to adapt to chronic effects of moderate levels of NH3. Future studies should explore acute effects of NH3 at higher levels on hen health and welfare.

KW - acute protein

KW - ammonia

KW - immunoglobulin

KW - inflammatory cytokine

KW - laying hen

UR - http://www.scopus.com/inward/record.url?scp=85037854102&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85037854102&partnerID=8YFLogxK

U2 - 10.3382/ps/pew454

DO - 10.3382/ps/pew454

M3 - Article

C2 - 28379573

AN - SCOPUS:85037854102

VL - 96

SP - 1524

EP - 1530

JO - Poultry Science

JF - Poultry Science

SN - 0032-5791

IS - 6

ER -