@article{54386bec36c247b8b822828d747d1e58,
title = "The effect of chronic ammonia exposure on acute-phase proteins, immunoglobulin, and cytokines in laying hens",
abstract = "Ammonia (NH3) is a potential health hazard to both humans and animals, causing local and systemic low-grade inflammation based on its levels and exposure durations. The objective of this study was to examine the effects of 45 wk of exposure to 30 ppm NH3 on the concentrations of acute-phase proteins, immunoglobulins, and cytokines in laying hens. At 18 wk of age, a group of Hy-Line W-36 hens was randomly assigned to 4-hen cages. These cages were evenly divided between 2 environmentally controlled chambers. At 25 wk of age, one chamber was maintained continuously with fresh air (NH3 < 5 ppm; control group) and the other one was injected with NH3 and controlled at 30 ppm (NH3 group) for 45 wk. At 70 wk of age, blood and spleen samples (n = 8 per treatment) were collected for analyses of immunological parameters. No significant differences were observed in plasma levels of albumin, complement components (C)-3 and C-4, immunoglobulin (Ig)M, IgA and IgG, interleukin (IL)-6, IL-10, or interferon gamma in the NH3 group compared to control group. Compared to control hens, NH3 exposed hens had higher plasma levels of α-1-acid glycoprotein (24%) and tumor necrosis factor alpha (54%) and higher mRNA expression of IL-1β (47%) and IL-6 (62.5%) in the spleen. These results indicated that hens may have the capability to adapt to chronic effects of moderate levels of NH3. Future studies should explore acute effects of NH3 at higher levels on hen health and welfare.",
keywords = "acute protein, ammonia, immunoglobulin, inflammatory cytokine, laying hen",
author = "Wu, {Y. N.} and Yan, {F. F.} and Hu, {J. Y.} and H. Chen and Tucker, {C. M.} and Green, {A. R.} and Cheng, {H. W.}",
note = "Funding Information: We would like to thank the staff and graduate students of the Agricultural Research Service, Livestock Behavior Research Unit and the Department of Animal Sciences of Purdue University and the Animal Welfare and Environmental Systems (AWES) Laboratory in the Department of Agricultural and Biological Engineering of University of Illinois for their contributions in conducting this study. Funding was provided by the National Institute of Food and Agriculture, U.S. Department of Agriculture under Award No. 2012-67021-19939. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement of the USDA. The USDA is an equal opportunity provider and employer. Funding Information: We would like to thank the staff and graduate students of the Agricultural Research Service, Livestock Behavior Research Unit and the Department of Animal Sciences of Purdue University and the Animal Welfare and Environmental Systems (AWES) Laboratory in the Department of Agricultural and Biological Engineering of University of Illinois for their contributions in conducting this study. Funding was provided by the National Institute of Food and Agriculture, U.S. Department of Agriculture under Award No. 2012-67021-19939 . Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement of the USDA. The USDA is an equal opportunity provider and employer. Publisher Copyright: {\textcopyright} 2017 Poultry Science Association Inc.",
year = "2017",
month = jun,
day = "1",
doi = "10.3382/ps/pew454",
language = "English (US)",
volume = "96",
pages = "1524--1530",
journal = "Poultry science",
issn = "0032-5791",
publisher = "Poultry Science Association",
number = "6",
}