The effect of assay composition, detergent solubilization and reconstitution on red beet (Beta vulgaris L.) plasma membrane H+-ATPase kinetic properties

John L. Giannini, Donald P. Briskin

Research output: Contribution to journalArticle

Abstract

Modification of the salt concentration, composition and/or buffer type in the assay of plasma membrane ATPase activity caused substantial changes in the Km and slight changes in the temperature dependence of this enzyme. The Km and temperature dependence were also affected by detergent solubilization of the ATPase and its subsequent reconstitution into liposomes. Modulation of kinetic properties by assay composition and hydrophobic state reflect the sensitivity of the plasma membrane H+-ATPase to its immediate environment. This may indicate a possible regulatory mechanism for this important plant enzyme.

Original languageEnglish (US)
Pages (from-to)189-193
Number of pages5
JournalPlant Science
Volume60
Issue number2
DOIs
StatePublished - 1989

Keywords

  • ATPase
  • detergent solubilization
  • plasma membrane
  • reconstitution

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

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