The Drosophila Duox maturation factor is a key component of a positive feedback loop that sustains regeneration signaling

Sumbul Jawed Khan, Syeda Nayab Fatima Abidi, Andrea Skinner, Yuan Tian, Rachel K. Smith-Bolton

Research output: Contribution to journalArticle

Abstract

Regenerating tissue must initiate the signaling that drives regenerative growth, and sustain that signaling long enough for regeneration to complete. How these key signals are sustained is unclear. To gain a comprehensive view of the changes in gene expression that occur during regeneration, we performed whole-genome mRNAseq of actively regenerating tissue from damaged Drosophila wing imaginal discs. We used genetic tools to ablate the wing primordium to induce regeneration, and carried out transcriptional profiling of the regeneration blastema by fluorescently labeling and sorting the blastema cells, thus identifying differentially expressed genes. Importantly, by using genetic mutants of several of these differentially expressed genes we have confirmed that they have roles in regeneration. Using this approach, we show that high expression of the gene moladietz (mol), which encodes the Duox-maturation factor NIP, is required during regeneration to produce reactive oxygen species (ROS), which in turn sustain JNK signaling during regeneration. We also show that JNK signaling upregulates mol expression, thereby activating a positive feedback signal that ensures the prolonged JNK activation required for regenerative growth. Thus, by whole-genome transcriptional profiling of regenerating tissue we have identified a positive feedback loop that regulates the extent of regenerative growth.

Original languageEnglish (US)
Article numbere1006937
JournalPLoS genetics
Volume13
Issue number7
DOIs
StatePublished - Jul 2017

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

Fingerprint Dive into the research topics of 'The Drosophila Duox maturation factor is a key component of a positive feedback loop that sustains regeneration signaling'. Together they form a unique fingerprint.

  • Cite this