The cytosolic G-protein α-subunit in human neutrophils responds to treatment with guanine nucleotides and magnesium

Uwe Rudolph, Günter Schultz, Walter Rosenthal

Research output: Contribution to journalArticlepeer-review

Abstract

The hydrodynamic properties of the Gi2 α-subunit in human neutrophil cytosol were analyzed. The s20,w value was 3.3 S at 30°C and 4.1 S at 4°C. Reconstitution of the cytosolic α-subunit with βγ-complex purified from porcine brain resulted in an s20,w value of 4.0 S at 30°C and 4.2 S at 4°C. Treatment of cytosol with G-protein-activating agents, GTPγS and MgCl2, decreased the s20,w value to 2.4 S at 30°C and 2.9 S at 4°C. Our results indicate that the cytosolic α-subunit of neutrophils is an inactive monomeric species at 30°C capable of interacting with G-protein βγ-complex and responsive to treatment with activating agents leading to the two activated forms, α* at 4°C and α** at 30°C.

Original languageEnglish (US)
Pages (from-to)137-142
Number of pages6
JournalFEBS Letters
Volume251
Issue number1-2
DOIs
StatePublished - Jul 17 1989
Externally publishedYes

Keywords

  • Cytosol
  • Guanine nucleotide
  • Guanine nucleotide binding regulatory protein
  • Neutrophil
  • Signal transduction

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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