The cytochrome P450 gene family CYP157 does not contain EXXR in the K-helix reducing the absolute conserved P450 residues to a single cysteine

Sanjeewa Rupasinghe, Mary A Schuler, Norio Kagawa, Hang Yuan, Li Lei, Bin Zhao, Steven L. Kelly, Michael R. Waterman, David C. Lamb

Research output: Contribution to journalArticle

Abstract

In this work, we have spectroscopically characterised CYP157C1 from Streptomyces coelicolor A3(2) which has the motif E 297 QSLW 301 rather than the invariant EXXR motif in the P450 K-helix. Site-directed mutagenesis of native E 297 QSLW 301 in CYP157C1 to E 297 ESLR 301 or E 297 QSRW 301 both containing standard EXXR motifs produced cytochrome P420 proteins thought to be inactive forms of P450 even though wild type CYP157C1 has the spectral properties of a normal P450. These results indicate that the EXXR motif is not required in all CYP tertiary architectures and only a single cysteine residue, which coordinates as the fifth thiolate ligand to the P450 haem iron, is invariant in all CYPs structures.

Original languageEnglish (US)
Pages (from-to)6338-6342
Number of pages5
JournalFEBS Letters
Volume580
Issue number27
DOIs
StatePublished - Nov 27 2006

Fingerprint

Streptomyces coelicolor
Mutagenesis
Site-Directed Mutagenesis
Heme
Cytochrome P-450 Enzyme System
Cysteine
Iron
Genes
Ligands
Proteins
cytochrome P420

Keywords

  • Cytochrome P450
  • Protein folding
  • Site-directed mutagenesis
  • Streptomyces

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

The cytochrome P450 gene family CYP157 does not contain EXXR in the K-helix reducing the absolute conserved P450 residues to a single cysteine. / Rupasinghe, Sanjeewa; Schuler, Mary A; Kagawa, Norio; Yuan, Hang; Lei, Li; Zhao, Bin; Kelly, Steven L.; Waterman, Michael R.; Lamb, David C.

In: FEBS Letters, Vol. 580, No. 27, 27.11.2006, p. 6338-6342.

Research output: Contribution to journalArticle

Rupasinghe, Sanjeewa ; Schuler, Mary A ; Kagawa, Norio ; Yuan, Hang ; Lei, Li ; Zhao, Bin ; Kelly, Steven L. ; Waterman, Michael R. ; Lamb, David C. / The cytochrome P450 gene family CYP157 does not contain EXXR in the K-helix reducing the absolute conserved P450 residues to a single cysteine. In: FEBS Letters. 2006 ; Vol. 580, No. 27. pp. 6338-6342.
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AU - Kagawa, Norio

AU - Yuan, Hang

AU - Lei, Li

AU - Zhao, Bin

AU - Kelly, Steven L.

AU - Waterman, Michael R.

AU - Lamb, David C.

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AB - In this work, we have spectroscopically characterised CYP157C1 from Streptomyces coelicolor A3(2) which has the motif E 297 QSLW 301 rather than the invariant EXXR motif in the P450 K-helix. Site-directed mutagenesis of native E 297 QSLW 301 in CYP157C1 to E 297 ESLR 301 or E 297 QSRW 301 both containing standard EXXR motifs produced cytochrome P420 proteins thought to be inactive forms of P450 even though wild type CYP157C1 has the spectral properties of a normal P450. These results indicate that the EXXR motif is not required in all CYP tertiary architectures and only a single cysteine residue, which coordinates as the fifth thiolate ligand to the P450 haem iron, is invariant in all CYPs structures.

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