The COP9 signalosome controls jasmonic acid synthesis and plant responses to herbivory and pathogens

Sarah R. Hind, Sarah E. Pulliam, Paola Veronese, Deepak Shantharaj, Azka Nazir, Nekaiya S. Jacobs, Johannes W. Stratmann

Research output: Contribution to journalArticle

Abstract

The COP9 signalosome (CSN) is a multi-protein complex that regulates the activities of cullin-RING E3 ubiquitin ligases (CRLs). CRLs ubiquitinate proteins in order to target them for proteasomal degradation. The CSN is required for proper plant development. Here we show that the CSN also has a profound effect on plant defense responses. Silencing of genes for CSN subunits in tomato plants resulted in a mild morphological phenotype and reduced expression of wound-responsive genes in response to mechanical wounding, attack by Manduca sexta larvae, and Prosystemin over-expression. In contrast, expression of pathogenesis-related genes was increased in a stimulus-independent manner in these plants. The reduced wound response in CSN-silenced plants corresponded with reduced synthesis of jasmonic acid (JA), but levels of salicylic acid (SA) were unaltered. As a consequence, these plants exhibited reduced resistance against herbivorous M. sexta larvae and the necrotrophic fungal pathogen Botrytis cinerea. In contrast, susceptibility to tobacco mosaic virus (TMV) was not altered in CSN-silenced plants. These data demonstrate that the CSN orchestrates not only plant development but also JA-dependent plant defense responses.

Original languageEnglish (US)
Pages (from-to)480-491
Number of pages12
JournalPlant Journal
Volume65
Issue number3
DOIs
StatePublished - Feb 1 2011
Externally publishedYes

Keywords

  • COP9 signalosome
  • jasmonic acid
  • plant-insect interactions
  • plant-pathogen interactions
  • systemin
  • virus-induced gene silencing

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

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  • Cite this

    Hind, S. R., Pulliam, S. E., Veronese, P., Shantharaj, D., Nazir, A., Jacobs, N. S., & Stratmann, J. W. (2011). The COP9 signalosome controls jasmonic acid synthesis and plant responses to herbivory and pathogens. Plant Journal, 65(3), 480-491. https://doi.org/10.1111/j.1365-313X.2010.04437.x