The cloning and expression of matrix metalloproteinase-2 and tissue inhibitor of matrix metalloproteinase 2 in normal canine lymph nodes and in canine lymphoma

R. G. Newman, B. E. Kitchell, M. A. Wallig, B. Paria

Research output: Contribution to journalArticlepeer-review

Abstract

Matrix metalloproteinase-2 (MMP-2) and its inhibitor, tissue inhibitor of matrix metalloproteinase 2 (TIMP2), are known to be important in cancer. The purposes of this study were to determine the cDNA sequence of canine MMP-2 and to investigate the expression patterns of MMP-2 and TIMP2 in normal canine lymph nodes and spontaneously arising canine lymphomas. We cloned and sequenced a PCR product containing most (1901 base pairs) of the coding sequence of canine MMP-2 that translates into a 623 amino acid protein. The cDNA and deduced amino acid sequences are highly homologous to those of other mammalian species. Canine MMP-2 and TIMP2 mRNAs were detectable in the majority of normal lymph node and lymphomatous samples evaluated. No statistical difference was identified when comparing the expression of either gene with regard to normal versus neoplastic nodes, nodal versus extranodal lymphoma, lymphoma grade, or B versus T cell immunophenotype.

Original languageEnglish (US)
Pages (from-to)206-214
Number of pages9
JournalResearch in Veterinary Science
Volume84
Issue number2
DOIs
StatePublished - Apr 2008

Keywords

  • Canine
  • Dog
  • Lymphoma
  • Matrix metalloproteinase 2
  • TIMP2

ASJC Scopus subject areas

  • veterinary(all)

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