TY - JOUR
T1 - The bovine herpesvirus type 1 U(L)3.5 Open reading frame encodes a virion structural protein
AU - Schikora, Beate
AU - Lu, Zhiqiang
AU - Kutish, Gerald F.
AU - Rock, Daniel
AU - Magyar, Gábor
AU - Letchworth, Geoffrey J.
N1 - The pSD72 plasmid was generously supplied by C. Whetstone. We thank the Letchworth lab, especially Peter S. Evans for his suggestions in confocal microscopy. We also thank Tamas Gaal for technical suggestions, Lee R. Martin for critical reading of the manu- script, and Hanns Ludwig for encouragement. This work was funded by Animal Health Grant 3822, USDA National Research Initiative Grant 92-37204-7900, and a Milwaukee Foundation Shaw Scholarship to G.J.L.
PY - 1998/1/5
Y1 - 1998/1/5
N2 - The bovine herpesvirus type 1 (BHV-1) open reading frame (ORF) U(L)3.5 is similar to ORFs found in pseudorabies virus, infectious laryngotracheitis virus, equine herpesvirus type 1, and varicella zoster virus, but clearly absent from herpes simplex virus. The published sequence for this ORF predicts a 126-amino-acid (13.2 kDa) protein product with an isoelectric point of 12.3. We confirmed the U(L)3.5 sequence, expressed the ORF as a glutathione-S-transferase fusion protein, and made rabbit antibodies against the purified fusion protein. The antiserum detected a 13-kDa protein in Western blots of MDBK cells infected with BHV-1, but not with other herpesviruses or uninfected cells. The BHV-1 U(L)3.5 protein was characterized as a component of the virion envelope or tegument because it was expressed as a late protein, it was present in the cytoplasm but not the nucleus of infected cells, and it was removed from purified virions by detergent extraction.
AB - The bovine herpesvirus type 1 (BHV-1) open reading frame (ORF) U(L)3.5 is similar to ORFs found in pseudorabies virus, infectious laryngotracheitis virus, equine herpesvirus type 1, and varicella zoster virus, but clearly absent from herpes simplex virus. The published sequence for this ORF predicts a 126-amino-acid (13.2 kDa) protein product with an isoelectric point of 12.3. We confirmed the U(L)3.5 sequence, expressed the ORF as a glutathione-S-transferase fusion protein, and made rabbit antibodies against the purified fusion protein. The antiserum detected a 13-kDa protein in Western blots of MDBK cells infected with BHV-1, but not with other herpesviruses or uninfected cells. The BHV-1 U(L)3.5 protein was characterized as a component of the virion envelope or tegument because it was expressed as a late protein, it was present in the cytoplasm but not the nucleus of infected cells, and it was removed from purified virions by detergent extraction.
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U2 - 10.1006/viro.1997.8918
DO - 10.1006/viro.1997.8918
M3 - Article
C2 - 9448691
AN - SCOPUS:0032484480
SN - 0042-6822
VL - 240
SP - 76
EP - 82
JO - Virology
JF - Virology
IS - 1
ER -