The Botrytis cinerea type III polyketide synthase shows unprecedented high catalytic efficiency toward long chain acyl-CoAs

Marimuthu Jeya, Tae Su Kim, Manish Kumar Tiwari, Jinglin Li, Huimin Zhao, Jung Kul Lee

Research output: Contribution to journalArticlepeer-review

Abstract

BPKS from Botrytis cinerea is a novel type III polyketide synthase that accepts C 4-C 18 aliphatic acyl-CoAs and benzoyl-CoA as the starters to form pyrones, resorcylic acids and resorcinols through sequential condensation with malonyl-CoA. The catalytic efficiency (k cat/K m) of BPKS was 2.8 × 10 5 s -1 M -1 for palmitoyl-CoA, the highest ever reported. Substrate docking analyses addressed the unique features of BPKS such as its high activity and high specificity toward long chain acyl-CoAs.

Original languageEnglish (US)
Pages (from-to)2864-2867
Number of pages4
JournalMolecular BioSystems
Volume8
Issue number11
DOIs
StatePublished - Nov 1 2012

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

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