The Biochemistry and Structural Biology of Cyanobactin Pathways: Enabling Combinatorial Biosynthesis

Wenjia Gu, Shi Hui Dong, Snigdha Sarkar, Satish K. Nair, Eric W. Schmidt

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Cyanobactin biosynthetic enzymes have exceptional versatility in the synthesis of natural and unnatural products. Cyanobactins are ribosomally synthesized and posttranslationally modified peptides synthesized by multistep pathways involving a broad suite of enzymes, including heterocyclases/cyclodehydratases, macrocyclases, proteases, prenyltransferases, methyltransferases, and others. Here, we describe the enzymology and structural biology of cyanobactin biosynthetic enzymes, aiming at the twin goals of understanding biochemical mechanisms and biosynthetic plasticity. We highlight how this common suite of enzymes may be utilized to generate a large array or structurally and chemically diverse compounds.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
EditorsBradley S. Moore
PublisherAcademic Press Inc.
Pages113-163
Number of pages51
ISBN (Print)9780128139592
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Enzymology
Volume604
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

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Keywords

  • Combinatorial biosynthesis
  • Cyanobactins
  • Cyclic peptide
  • Natural products
  • RiPP biosynthesis
  • Synthetic biology

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Gu, W., Dong, S. H., Sarkar, S., Nair, S. K., & Schmidt, E. W. (2018). The Biochemistry and Structural Biology of Cyanobactin Pathways: Enabling Combinatorial Biosynthesis. In B. S. Moore (Ed.), Methods in Enzymology (pp. 113-163). (Methods in Enzymology; Vol. 604). Academic Press Inc.. https://doi.org/10.1016/bs.mie.2018.03.002