The biochemical mechanisms of the plant activation of promutagenic aromatic amines

Elizabeth D. Wagner, Mary M. Verdier, Michael Jacob Plewa

Research output: Contribution to journalArticle

Abstract

Using specific monooxygenase and oxidase inhibitors in a plant cell/microbe coincubation assay, the biochemical mechanisms of the plant activation of two aromatic amines were compared. The biological endpoints included mutation induction, inhibition of mutagenicity, viability of the plant cells (activating system), and viability of the microbial cells (genetic indicator organism). The activation of m‐phenylenediamine by TX1 cells was mediated by enzyme systems that were inhibited by diethyldithiocarbamate, potassium cyanide, methimazole, ( + )‐catechin or acetaminophen. The inhibition by metyrapone was attended by toxicity in the plant cells. These data implicate a TX1 cell peroxidase and a FAD‐dependent monooxygenase in the plant activation of m‐phenylenediamine. The TX1 cell activation of 2‐aminofluorene was inhibited by diethyldithiocarbamate, 7,8‐benzoflavone, acetaminophen or ( + )‐catechin. An additional pathway of the plant cells in the activation of 2‐aminofluorene may involve a cytochrome P‐448‐type N‐hydroxylase.

Original languageEnglish (US)
Pages (from-to)236-244
Number of pages9
JournalEnvironmental and Molecular Mutagenesis
Volume15
Issue number4
DOIs
StatePublished - 1990

Fingerprint

Plant Cells
Amines
Ditiocarb
Catechin
Acetaminophen
Mixed Function Oxygenases
Microbial Genetics
Potassium Cyanide
Methimazole
Metyrapone
Cytochromes
Peroxidase
Cell Survival
Oxidoreductases
Mutation
Enzymes

Keywords

  • 2‐aminofluorene
  • antimutagenesis
  • monooxygenase
  • m‐phenylenediamine
  • oxidase inhibitors

ASJC Scopus subject areas

  • Epidemiology
  • Genetics(clinical)
  • Health, Toxicology and Mutagenesis

Cite this

The biochemical mechanisms of the plant activation of promutagenic aromatic amines. / Wagner, Elizabeth D.; Verdier, Mary M.; Plewa, Michael Jacob.

In: Environmental and Molecular Mutagenesis, Vol. 15, No. 4, 1990, p. 236-244.

Research output: Contribution to journalArticle

Wagner, Elizabeth D. ; Verdier, Mary M. ; Plewa, Michael Jacob. / The biochemical mechanisms of the plant activation of promutagenic aromatic amines. In: Environmental and Molecular Mutagenesis. 1990 ; Vol. 15, No. 4. pp. 236-244.
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