The bc1 complexes of Rhodobacter sphaeroides and Rhodobacter capsulatus

Robert B. Gennis, Blanca Barquera, Beth Hacker, Steven R. Van Doren, Sylvain Arnaud, Antony R. Crofts, Edgar Davidson, Kevin A. Gray, Fevzi Daldal

Research output: Contribution to journalArticle

Abstract

Photosynthetic bacteria offer excellent experimental opportunities to explore both the structure and function of the ubiquinol-cytochrome c oxidoreductase (bc1 complex). In both Rhodobacter sphaeroides and Rhodobacter capsulatus, the bc1 complex functions in both the aerobic respiratory chain and as an essential component of the photosynthetic electron transport chain. Because the bc1 complex in these organisms can be functionally coupled to the photosynthetic reaction center, flash photolysis can be used to study electron flow through the enzyme and to examine the effects of various amino acid substitutions. During the past several years, numerous mutations have been generated in the cytochrome b subunit, in the Rieske iron-sulfur subunit, and in the cytochrome c1 subunit. Both site-directed and random mutagenesis procedures have been utilized. Studies of these mutations have identified amino acid residues that are metal ligands, as well as those residues that are at or near either the quinol oxidase (Qo) site or the quinol reductase (Qi) site. The postulate that these two Q-sites are located on opposite sides of the membrane is supported by these studies. Current research is directed at exploring the details of the catalytic mechanism, the nature of the subunit interactions, and the assembly of this enzyme.

Original languageEnglish (US)
Pages (from-to)195-209
Number of pages15
JournalJournal of Bioenergetics and Biomembranes
Volume25
Issue number3
DOIs
StatePublished - Jun 1 1993

Keywords

  • Cytochrome
  • Q-cycle
  • bc
  • complex III
  • photosynthesis

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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    Gennis, R. B., Barquera, B., Hacker, B., Van Doren, S. R., Arnaud, S., Crofts, A. R., Davidson, E., Gray, K. A., & Daldal, F. (1993). The bc1 complexes of Rhodobacter sphaeroides and Rhodobacter capsulatus. Journal of Bioenergetics and Biomembranes, 25(3), 195-209. https://doi.org/10.1007/BF00762582