The Bacteroides thetaiotaomicron protein bacteroides host factor A participates in integration of the integrative conjugative element CTnDOT into the chromosome

Kenneth Ringwald, Jeffrey Gardner

Research output: Contribution to journalArticle

Abstract

CTnDOT is a conjugative transposon found in Bacteroides species. It encodes multiple antibiotic resistances and is stimulated to transfer by exposure to tetracycline. CTnDOT integration into the host chromosome requires IntDOT and a previously unknown host factor. We have identified a protein, designated BHFa (Bacteroides host factor A), that participates in integrative recombination. BHFa is the first host factor identified for a site-specific recombination reaction in the CTnDOT family of integrative and conjugative elements. Based on the amino acid sequence of BHFa, the ability to bind specifically to 4 sites in the attDOT DNA, and its activity in the integration reaction, BHFa is a member of the IHF/HU family of nucleoid-associated proteins. Other DNA bending proteins that bind DNA nonspecifically can substitute for BHFa in the integration reaction.

Original languageEnglish (US)
Pages (from-to)1339-1349
Number of pages11
JournalJournal of bacteriology
Volume197
Issue number8
DOIs
StatePublished - Jan 1 2015

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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