The anticancer potential of steroidal saponin, dioscin, isolated from wild yam (Dioscorea villosa) root extract in invasive human breast cancer cell line MDA-MB-231 in vitro

Pranapda Aumsuwan, Shabana I. Khan, Ikhlas A. Khan, Zulfiqar Ali, Bharathi Avula, Larry A. Walker, Zia Shariat-Madar, William G Helferich, Benita S Katzenellenbogen, Asok K. Dasmahapatra

Research output: Contribution to journalArticle

Abstract

Previously, we observed that wild yam (Dioscorea villosa) root extract (WYRE) was able to activate GATA3 in human breast cancer cells targeting epigenome. This study aimed to find out if dioscin (DS), a bioactive compound of WYRE, can modulate GATA3 functions and cellular invasion in human breast cancer cells. MCF-7 and MDA-MB-231 cells were treated in the absence/presence of various concentrations of DS and subjected to gene analysis by RT-qPCR, immunoblotting, and immunocytochemistry. We determined the ability of MDA-MB-231 cells to migrate into wound area and examined the effects of DS on cellular invasion using invasion assay. DS reduced cell viability of both cell lines in a concentration and time-dependent manner. GATA3 expression was enhanced by DS (5.76 μM) in MDA-MB-231 cells. DS (5.76 μM)-treated MDA-MB-231 cells exhibited the morphological characteristic of epithelial-like cells; mRNA expression of DNMT3A, TET2, TET3, ZFPM2 and E-cad were increased while TET1, VIM and MMP9 were decreased. Cellular invasion of MDA-MB-231 was reduced by 65 ± 5% in the presence of 5.76 μM DS. Our data suggested that DS-mediated pathway could promote GATA3 expression at transcription and translation levels. We propose that DS has potential to be used as an anti-invasive agent in breast cancer.

Original languageEnglish (US)
Pages (from-to)98-110
Number of pages13
JournalArchives of Biochemistry and Biophysics
Volume591
DOIs
StatePublished - Feb 1 2016

Fingerprint

Dioscorea
Saponins
Cells
Breast Neoplasms
Cell Line
In Vitro Techniques
dioscin
Transcription
Immunoblotting
Assays
Cell Survival
Genes
Epithelial Cells
Immunohistochemistry
Messenger RNA

Keywords

  • Dioscin
  • GATA3
  • Gene expression
  • Invasion/migration
  • ZFPM2

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

The anticancer potential of steroidal saponin, dioscin, isolated from wild yam (Dioscorea villosa) root extract in invasive human breast cancer cell line MDA-MB-231 in vitro. / Aumsuwan, Pranapda; Khan, Shabana I.; Khan, Ikhlas A.; Ali, Zulfiqar; Avula, Bharathi; Walker, Larry A.; Shariat-Madar, Zia; Helferich, William G; Katzenellenbogen, Benita S; Dasmahapatra, Asok K.

In: Archives of Biochemistry and Biophysics, Vol. 591, 01.02.2016, p. 98-110.

Research output: Contribution to journalArticle

Aumsuwan, Pranapda ; Khan, Shabana I. ; Khan, Ikhlas A. ; Ali, Zulfiqar ; Avula, Bharathi ; Walker, Larry A. ; Shariat-Madar, Zia ; Helferich, William G ; Katzenellenbogen, Benita S ; Dasmahapatra, Asok K. / The anticancer potential of steroidal saponin, dioscin, isolated from wild yam (Dioscorea villosa) root extract in invasive human breast cancer cell line MDA-MB-231 in vitro. In: Archives of Biochemistry and Biophysics. 2016 ; Vol. 591. pp. 98-110.
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abstract = "Previously, we observed that wild yam (Dioscorea villosa) root extract (WYRE) was able to activate GATA3 in human breast cancer cells targeting epigenome. This study aimed to find out if dioscin (DS), a bioactive compound of WYRE, can modulate GATA3 functions and cellular invasion in human breast cancer cells. MCF-7 and MDA-MB-231 cells were treated in the absence/presence of various concentrations of DS and subjected to gene analysis by RT-qPCR, immunoblotting, and immunocytochemistry. We determined the ability of MDA-MB-231 cells to migrate into wound area and examined the effects of DS on cellular invasion using invasion assay. DS reduced cell viability of both cell lines in a concentration and time-dependent manner. GATA3 expression was enhanced by DS (5.76 μM) in MDA-MB-231 cells. DS (5.76 μM)-treated MDA-MB-231 cells exhibited the morphological characteristic of epithelial-like cells; mRNA expression of DNMT3A, TET2, TET3, ZFPM2 and E-cad were increased while TET1, VIM and MMP9 were decreased. Cellular invasion of MDA-MB-231 was reduced by 65 ± 5{\%} in the presence of 5.76 μM DS. Our data suggested that DS-mediated pathway could promote GATA3 expression at transcription and translation levels. We propose that DS has potential to be used as an anti-invasive agent in breast cancer.",
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AU - Khan, Shabana I.

AU - Khan, Ikhlas A.

AU - Ali, Zulfiqar

AU - Avula, Bharathi

AU - Walker, Larry A.

AU - Shariat-Madar, Zia

AU - Helferich, William G

AU - Katzenellenbogen, Benita S

AU - Dasmahapatra, Asok K.

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