TY - JOUR
T1 - The anaerobic proteins of maize
AU - Sachs, Martin M.
AU - Freeling, Michael
AU - Okimoto, Ronald
N1 - Funding Information:
We thank Robert J. Ferl for suggesting the use of high concentrations of 2-mercaptoethanol in the extraction buffer, and Andrei Laszlo for suggesting the use of glycerol in the native gels. We also thank David S. K. Cheng and Deverie Bongard for help with the figures. M. M. S. is the recipient of a USPHS predoctoral grant. This research was supported by an NIH grant.
PY - 1980
Y1 - 1980
N2 - Anaerobic treatment drastically alters the pattern of protein synthesized by maize primary roots. During the first hour of anaerobiosis, aerobic protein synthesis is halted and there is an increase in the synthesis of a class of polypeptides with approximate molecular weights of 33,000 daltons. During the second hour of anaerobic treatment, the synthesis of another small group of polypeptides is initiated. This group, the anaerobic polypeptides (ANPs), accounts for 〉70% of total protein synthesis after 5 hr of anaerobiosis, and is synthesized in basically the same ratio until root death (∼70 hr). The alcohol dehydrogenase polypeptides are major ANPs. RNA isolated from roots treated anaerobically for at least 24 hr directs the translation of only the anaerobic polypeptides. However, RNA from roots treated anaerobically for only 5 hr directs translation of both anaerobic and aerobic polypeptides. Thus an early response to anaerobic treatment is the suppression of aerobic message translation. Although the anaerobic polypeptides share a formal similarity to heat-shock proteins in animals, it is probable that the anaerobic genes are an adaptation to flooding.
AB - Anaerobic treatment drastically alters the pattern of protein synthesized by maize primary roots. During the first hour of anaerobiosis, aerobic protein synthesis is halted and there is an increase in the synthesis of a class of polypeptides with approximate molecular weights of 33,000 daltons. During the second hour of anaerobic treatment, the synthesis of another small group of polypeptides is initiated. This group, the anaerobic polypeptides (ANPs), accounts for 〉70% of total protein synthesis after 5 hr of anaerobiosis, and is synthesized in basically the same ratio until root death (∼70 hr). The alcohol dehydrogenase polypeptides are major ANPs. RNA isolated from roots treated anaerobically for at least 24 hr directs the translation of only the anaerobic polypeptides. However, RNA from roots treated anaerobically for only 5 hr directs translation of both anaerobic and aerobic polypeptides. Thus an early response to anaerobic treatment is the suppression of aerobic message translation. Although the anaerobic polypeptides share a formal similarity to heat-shock proteins in animals, it is probable that the anaerobic genes are an adaptation to flooding.
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U2 - 10.1016/0092-8674(80)90322-0
DO - 10.1016/0092-8674(80)90322-0
M3 - Article
C2 - 7418006
AN - SCOPUS:0018869288
SN - 0092-8674
VL - 20
SP - 761
EP - 767
JO - Cell
JF - Cell
IS - 3
ER -