The addition of the amniotic membrane to mineralized collagen scaffolds for immunomodulatory bone regeneration

Marley J. Dewey, Eileen M. Johnson, Simona Slater, Brendan A Harley

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Statement of Purpose: Cranio-maxillofacial (CMF) defects often are comprised of critical sized bone defects that will not heal without surgical intervention. Examples of CMF defects extend from birth defects, to post-oncologic treatment and high-energy impacts, with billions spent on repair. Due to the overwhelmingly large portion of bone missing in these defects, there is a high chance for infection and a high probability of chronic inflammation occurring. This leaves a crucial niche for developing a bone regenerative material that can modulate the immune response to aid healing. Efforts in our lab have recently developed a mineralized collagen-GAG scaffold to induce MSC osteogenic differentiation and CMF bone regeneration in the absence of traditional pro-osteogenic signals [1]. Here we describe the incorporation of the amniotic membrane, known for its anti-inflammatory, anti-scarring, and anti-microbial properties, into mineralized collagen scaffolds for immunomodulatory applications. We examine the mechanical properties and pore size upon the addition of the amniotic membrane, as well as the response of porcine adipose derived stem cells (pASCs) seeded on these scaffolds in both normal media and high inflammatory media. We evaluate the metabolic activity, cell number, gene expression, protein activity, and mineralization of these scaffolds in vitro. Current work explores the bacterial response on the amniotic membrane and macrophage polarization.

Original languageEnglish (US)
Title of host publicationSociety for Biomaterials Annual Meeting and Exposition 2019
Subtitle of host publicationThe Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting
PublisherSociety for Biomaterials
Number of pages1
ISBN (Electronic)9781510883901
StatePublished - Jan 1 2019
Event42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Seattle, United States
Duration: Apr 3 2019Apr 6 2019

Publication series

NameTransactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
Volume40
ISSN (Print)1526-7547

Conference

Conference42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
CountryUnited States
CitySeattle
Period4/3/194/6/19

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Biotechnology
  • Biomaterials
  • Materials Chemistry

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  • Cite this

    Dewey, M. J., Johnson, E. M., Slater, S., & Harley, B. A. (2019). The addition of the amniotic membrane to mineralized collagen scaffolds for immunomodulatory bone regeneration. In Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium; Vol. 40). Society for Biomaterials.