The respiration rate of Euglena in the presence of 2.0 μg. or 0.1 μg. testosterone/ml. medium is about 20 and 14% higher, respectively, than that of controls lacking the steroid. This result obtains whether or not the cells were previously grown in the presence of testosterone. Further, the increased respiration rate of Euglena in the presence of testosterone is dependent upon the presence of a nitrogen source. The testosterone "effect" is reduced when nitrogen is absent from the respiration medium and is restored when nitrogen is reintroduced into the medium. The data suggest that an early effect of this steroid on Euglena is on the permeability of these cells to nitrogen-containing compounds with a resultant effect on protein metabolism.
ASJC Scopus subject areas
- Molecular Biology