The actin filament bundling protein α-actinin-4 actually suppresses actin stress fibers by permitting actin turnover

James Peter Kemp, William M Brieher

Research output: Contribution to journalArticle

Abstract

Cells organize actin filaments into contractile bundles known as stress fibers that resist mechanical stress,increase cell adhesion, remodel the extracellular matrix,and maintain tissue integrity.α-actinin is an actin filament bundling protein that is thought to be essential for stress fiber formation and stability.However, previous studies have also suggested that α-actinin might disrupt fibers,making the true function of this biomolecule unclear. Here we use fluorescence imaging to show that kidney epithelial cells depleted of α-actinin-4 via shRNA or CRISPR/Cas9, or expressing a disruptive mutant make more massive stress fibers that are less dynamic than those in WT cells,leading to defects in cell motility and wound healing.The increase in stress fiber mass and stability can be explained, in part, by increased loading of the filament component tropomyosin onto stress fibers in the absence ofα-actinin, as monitored via immunofluorescence. We show using imaging and cosedimentation that α-actinin and tropomyosin compete for binding to F-actin and that tropomyosin shields actin filaments from cofilin-mediated disassembly in vitro and in cells. Perturbing tropomyosin in cells lacking α-actinin-4 results in a complete loss of stress fibers.Our results with α-actinin-4 on stress fiber organization are the opposite of what might have been predicted from previous in vitro biochemistry and further highlight how the complex interactions of multiple proteins competing for filament binding lead to unexpected functions for actin-binding proteins in cells.

Original languageEnglish (US)
Pages (from-to)14520-14533
Number of pages14
JournalJournal of Biological Chemistry
Volume293
Issue number37
DOIs
StatePublished - Jan 1 2018

Fingerprint

Actinin
Stress Fibers
Actins
Tropomyosin
Fibers
Actin Cytoskeleton
Clustered Regularly Interspaced Short Palindromic Repeats
Actin Depolymerizing Factors
Microfilament Proteins
Mechanical Stress
Imaging techniques
Optical Imaging
Biochemistry
actin filament bundling proteins
Cell adhesion
Cell Adhesion
Biomolecules
Wound Healing
Small Interfering RNA
Cell Movement

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

The actin filament bundling protein α-actinin-4 actually suppresses actin stress fibers by permitting actin turnover. / Kemp, James Peter; Brieher, William M.

In: Journal of Biological Chemistry, Vol. 293, No. 37, 01.01.2018, p. 14520-14533.

Research output: Contribution to journalArticle

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