The abundant 19‐kilodalton protein associated with human sperm nuclei that is related to seminal plasma α‐inhibins

Andrei O. Zalensky, Peter Yau, John W. Breneman, E. Morton Bradbury

Research output: Contribution to journalArticlepeer-review

Abstract

A basic protein with a relative molecular mass of 19 kDa has been identified and isolated to purity from sonication‐resistant, partially demembranized human sperm nuclei. Several criteria prove that this is the unique sperm‐specific protein, which was previously thought to be a sperm/testis histone. Partial primary structure sequencing demonstrates homologies with human seminal α‐inhibins and semenogelin. From the sequence and Western‐blotting data with antibodies against basic seminal inhibin‐like peptide, we propose that this 19‐kD protein is a product of 52‐kDa semenogelin processing. The 19‐kDa protein was not found among seminal plasma proteins and may be protected from further cleavage into inhibin‐like peptides by its association with the sperm head Immunofluorescence data indicate its localization in the nuclear periphery, with preferential concentration at the acrosome calyx boundary. © 1993 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)164-173
Number of pages10
JournalMolecular reproduction and development
Volume36
Issue number2
DOIs
StatePublished - Oct 1993
Externally publishedYes

Keywords

  • Basic proteins
  • Semenogelin processing
  • Sperm head

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

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