The 2b protein as a minor structural component of PRRSV

Wai Hong Wu; Ying Fang; Raymond R.R. Rowland; Steven R. Lawson; Jane Christopher-Hennings; Kyoung Jin Yoon; Eric A. Nelson

doi:10.1016/j.virusres.2005.06.014

The 2b protein as a minor structural component of PRRSV

Wai Hong Wu, Ying Fang, Raymond R.R. Rowland, Steven R. Lawson, Jane Christopher-Hennings, Kyoung Jin Yoon, Eric A. Nelson

Research output: Contribution to journal › Article › peer-review

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) ORF2 contains an internal ORF that codes for a small non-glycosylated protein known as 2b. Previous work had identified the presence of a 10 kDa 2b protein in virus-infected cells and the induction of an anti-2b response in PRRSV-infected pigs, as well as a possible association of 2b with the virion (Wu et al., 2001, Virology 287:183-191). In this study, we utilized two experimental approaches, including the use of a 2b peptide-specific monoclonal antibody, to demonstrate that the PRRSV 2b protein is an integral component of the PRRSV virion. This study suggests that 2b in PRRSV is similar to the E protein in EAV and forms a minor structural component of the virion.

Original language	English (US)
Pages (from-to)	177-181
Number of pages	5
Journal	Virus Research
Volume	114
Issue number	1-2
DOIs	https://doi.org/10.1016/j.virusres.2005.06.014
State	Published - Dec 2005
Externally published	Yes

Keywords

2b protein
Arterivirus
Porcine reproductive and respiratory syndrome virus (PRRSV)

ASJC Scopus subject areas

Cancer Research
Virology
Infectious Diseases

Online availability

10.1016/j.virusres.2005.06.014

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title = "The 2b protein as a minor structural component of PRRSV",

abstract = "Porcine reproductive and respiratory syndrome virus (PRRSV) ORF2 contains an internal ORF that codes for a small non-glycosylated protein known as 2b. Previous work had identified the presence of a 10 kDa 2b protein in virus-infected cells and the induction of an anti-2b response in PRRSV-infected pigs, as well as a possible association of 2b with the virion (Wu et al., 2001, Virology 287:183-191). In this study, we utilized two experimental approaches, including the use of a 2b peptide-specific monoclonal antibody, to demonstrate that the PRRSV 2b protein is an integral component of the PRRSV virion. This study suggests that 2b in PRRSV is similar to the E protein in EAV and forms a minor structural component of the virion.",

keywords = "2b protein, Arterivirus, Porcine reproductive and respiratory syndrome virus (PRRSV)",

author = "Wu, {Wai Hong} and Ying Fang and Rowland, {Raymond R.R.} and Lawson, {Steven R.} and Jane Christopher-Hennings and Yoon, {Kyoung Jin} and Nelson, {Eric A.}",

note = "Funding Information: This work was supported by the USDA National Research Initiative Competitive Grants Program Grants 9502254, 9702754, 0002114 and 2003-35204-13704; National Science Foundation Grant OSR-9108773, South Dakota Future Fund and the South Dakota Agricultural Experiment Station. We thank Dr. Ronald Magar for the generous gift of monoclonal antibody 2C12.",

year = "2005",

month = dec,

doi = "10.1016/j.virusres.2005.06.014",

language = "English (US)",

volume = "114",

pages = "177--181",

journal = "Virus Research",

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TY - JOUR

T1 - The 2b protein as a minor structural component of PRRSV

AU - Wu, Wai Hong

AU - Fang, Ying

AU - Rowland, Raymond R.R.

AU - Lawson, Steven R.

AU - Christopher-Hennings, Jane

AU - Yoon, Kyoung Jin

AU - Nelson, Eric A.

N1 - Funding Information: This work was supported by the USDA National Research Initiative Competitive Grants Program Grants 9502254, 9702754, 0002114 and 2003-35204-13704; National Science Foundation Grant OSR-9108773, South Dakota Future Fund and the South Dakota Agricultural Experiment Station. We thank Dr. Ronald Magar for the generous gift of monoclonal antibody 2C12.

PY - 2005/12

Y1 - 2005/12

N2 - Porcine reproductive and respiratory syndrome virus (PRRSV) ORF2 contains an internal ORF that codes for a small non-glycosylated protein known as 2b. Previous work had identified the presence of a 10 kDa 2b protein in virus-infected cells and the induction of an anti-2b response in PRRSV-infected pigs, as well as a possible association of 2b with the virion (Wu et al., 2001, Virology 287:183-191). In this study, we utilized two experimental approaches, including the use of a 2b peptide-specific monoclonal antibody, to demonstrate that the PRRSV 2b protein is an integral component of the PRRSV virion. This study suggests that 2b in PRRSV is similar to the E protein in EAV and forms a minor structural component of the virion.

AB - Porcine reproductive and respiratory syndrome virus (PRRSV) ORF2 contains an internal ORF that codes for a small non-glycosylated protein known as 2b. Previous work had identified the presence of a 10 kDa 2b protein in virus-infected cells and the induction of an anti-2b response in PRRSV-infected pigs, as well as a possible association of 2b with the virion (Wu et al., 2001, Virology 287:183-191). In this study, we utilized two experimental approaches, including the use of a 2b peptide-specific monoclonal antibody, to demonstrate that the PRRSV 2b protein is an integral component of the PRRSV virion. This study suggests that 2b in PRRSV is similar to the E protein in EAV and forms a minor structural component of the virion.

KW - 2b protein

KW - Arterivirus

KW - Porcine reproductive and respiratory syndrome virus (PRRSV)

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The 2b protein as a minor structural component of PRRSV

Abstract

Keywords

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