Temperature-mediated differential expression of immune and stress-related genes in Aedes aegypti Larvae

Ephantus J. Muturi, Alice Nyakeriga, Millon Blackshear

Research output: Contribution to journalArticlepeer-review

Abstract

The mechanisms by which natural populations of vector mosquitoes cope with daily and seasonal fluctuations in temperature are poorly understood. We examined the effect of water temperature on expression of stress and immune-related genes in Aedes aegypti larvae. Aedes aegypti 3rd instars were exposed for 24 h to one of 7 constant temperatures (10°C, 15°C, 20°C, 25°C control, 32°C, 36°C, or 40°C) and expression of antimicrobial peptides (cecropin, defensin), transferrin, and heat shock proteins (HSP70 and HSP83) quantified by real-time reverse-transcriptase polymerase chain reaction. Cecropin, defensin, and transferrin were overexpressed at 36°C and underexpressed at 15°C and 32°C. HSP83 was overexpressed at 10°C and 40°C and underexpressed at 20°C, while HSP70 was underexpressed at 15°C, 32°C, and 36°C. These findings suggest that antimicrobial peptides can serve as biomarkers of thermal stress and that HSP83 may buffer mosquito larvae against extreme temperatures.

Original languageEnglish (US)
Pages (from-to)79-83
Number of pages5
JournalJournal of the American Mosquito Control Association
Volume28
Issue number2
DOIs
StatePublished - Jun 1 2012

Keywords

  • Aedes aegypti
  • Temperature
  • gene expression

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Public Health, Environmental and Occupational Health
  • Insect Science

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