Temperature- and salt-dependent binding of long DNA to protein-sized quantum dots: Thermodynamics of 'inorganic protein' - DNA interactions

Rahina Mahtab, H. Hydrick Harden, Catherine J. Murphy

Research output: Contribution to journalArticle

Abstract

The adsorption of calf thymus DNA to 45 Å nanoparticles of Cd(II)-rich CdS has been examined by photoluminescence spectroscopy as a function of temperature. The resulting van't Hoff plot suggests that the driving force for adsorption is entropy, and the enthalpic contribution to DNA-surface binding is slightly unfavorable. A likely source of the increase in entropy upon binding is release of solvent and/or counterions from the interface, analogous to what has been observed for nonspecific protein-DNA interactions. Reverse salt titrations suggest that counterion release is a substantial component of the nanoparticle-DNA interaction.

Original languageEnglish (US)
Pages (from-to)14-17
Number of pages4
JournalJournal of the American Chemical Society
Volume122
Issue number1
DOIs
StatePublished - Jan 12 2000

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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