Techniques for chromosomal integration and expression optimization in Escherichia coli

Bingming Ou; Carolina Garcia; Yejun Wang; Weiping Zhang; Guoqiang Zhu

doi:10.1002/bit.26790

Techniques for chromosomal integration and expression optimization in Escherichia coli

Bingming Ou, Carolina Garcia, Yejun Wang, Weiping Zhang, Guoqiang Zhu

Research output: Contribution to journal › Review article › peer-review

Abstract

Due to the inherent expression stability and low metabolic burden to the host cell, the expression of heterologous proteins in the bacterial chromosome in a precise and efficient manner is highly desirable for metabolic engineering and live bacterial applications. However, obtaining suitable chromosome expression levels is particularly challenging. In this minireview, we briefly present the technologies available for the integration of heterologous genes into Escherichia coli chromosomes and strategies to optimize the expression levels of heterologous proteins.

Original language	English (US)
Pages (from-to)	2467-2478
Number of pages	12
Journal	Biotechnology and bioengineering
Volume	115
Issue number	10
DOIs	https://doi.org/10.1002/bit.26790
State	Published - Oct 2018
Externally published	Yes

Keywords

Escherichia coli (E. coli)
chromosomal expression
chromosomal integration
expression cassette design
optimization
synthetic biology

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

Online availability

10.1002/bit.26790

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title = "Techniques for chromosomal integration and expression optimization in Escherichia coli",

abstract = "Due to the inherent expression stability and low metabolic burden to the host cell, the expression of heterologous proteins in the bacterial chromosome in a precise and efficient manner is highly desirable for metabolic engineering and live bacterial applications. However, obtaining suitable chromosome expression levels is particularly challenging. In this minireview, we briefly present the technologies available for the integration of heterologous genes into Escherichia coli chromosomes and strategies to optimize the expression levels of heterologous proteins.",

keywords = "Escherichia coli (E. coli), chromosomal expression, chromosomal integration, expression cassette design, optimization, synthetic biology",

author = "Bingming Ou and Carolina Garcia and Yejun Wang and Weiping Zhang and Guoqiang Zhu",

note = "The authors thank the financial support for this study that was provided by the Science and Technology Department of Jiangsu Province (Grant no. BE2017342), the Chinese National Science Foundation (Grant nos. 31672579, 30571374, 30771603, 31072136, 31270171), supported by the Priority Academic Program of Development Jiangsu High Education Institution, and a project founded by the China scholarship council for studying in the Kansas State University. Science and Technology Department of Jiangsu Province, Grant/Award Number: BE2017342; Chinese National Science Foundation, Grant/Award Numbers: 31072136, 31270171, 30571374, 31672579, 30771603; Priority Academic Program of Development Jiangsu High Education Institution",

year = "2018",

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doi = "10.1002/bit.26790",

language = "English (US)",

volume = "115",

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journal = "Biotechnology and bioengineering",

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TY - JOUR

T1 - Techniques for chromosomal integration and expression optimization in Escherichia coli

AU - Ou, Bingming

AU - Garcia, Carolina

AU - Wang, Yejun

AU - Zhang, Weiping

AU - Zhu, Guoqiang

N1 - The authors thank the financial support for this study that was provided by the Science and Technology Department of Jiangsu Province (Grant no. BE2017342), the Chinese National Science Foundation (Grant nos. 31672579, 30571374, 30771603, 31072136, 31270171), supported by the Priority Academic Program of Development Jiangsu High Education Institution, and a project founded by the China scholarship council for studying in the Kansas State University. Science and Technology Department of Jiangsu Province, Grant/Award Number: BE2017342; Chinese National Science Foundation, Grant/Award Numbers: 31072136, 31270171, 30571374, 31672579, 30771603; Priority Academic Program of Development Jiangsu High Education Institution

PY - 2018/10

Y1 - 2018/10

N2 - Due to the inherent expression stability and low metabolic burden to the host cell, the expression of heterologous proteins in the bacterial chromosome in a precise and efficient manner is highly desirable for metabolic engineering and live bacterial applications. However, obtaining suitable chromosome expression levels is particularly challenging. In this minireview, we briefly present the technologies available for the integration of heterologous genes into Escherichia coli chromosomes and strategies to optimize the expression levels of heterologous proteins.

AB - Due to the inherent expression stability and low metabolic burden to the host cell, the expression of heterologous proteins in the bacterial chromosome in a precise and efficient manner is highly desirable for metabolic engineering and live bacterial applications. However, obtaining suitable chromosome expression levels is particularly challenging. In this minireview, we briefly present the technologies available for the integration of heterologous genes into Escherichia coli chromosomes and strategies to optimize the expression levels of heterologous proteins.

KW - Escherichia coli (E. coli)

KW - chromosomal expression

KW - chromosomal integration

KW - expression cassette design

KW - optimization

KW - synthetic biology

UR - https://www.scopus.com/pages/publications/85052507086

UR - https://www.scopus.com/inward/citedby.url?scp=85052507086&partnerID=8YFLogxK

U2 - 10.1002/bit.26790

DO - 10.1002/bit.26790

M3 - Review article

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AN - SCOPUS:85052507086

SN - 0006-3592

VL - 115

SP - 2467

EP - 2478

JO - Biotechnology and bioengineering

JF - Biotechnology and bioengineering

IS - 10

ER -

Techniques for chromosomal integration and expression optimization in Escherichia coli

Abstract

Keywords

ASJC Scopus subject areas

Online availability

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