TY - GEN
T1 - Technique for false image correction in second harmonic generation microscopy by modulating laser polarization
AU - Jin, L.
AU - Gao, L.
AU - Huo, L.
AU - Yuan, W.
AU - Luo, Y. H.
AU - Hot, A. H.P.
AU - Lint, C.
N1 - Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2006
Y1 - 2006
N2 - Second harmonic generation microscopy (SHGM) has been widely used in biomedicine as a promising non-invasive imaging technique. However, due to the complexity of biological samples, false images are often produced depending on sample orientation relative to laser polarization and on sample parameter related to the second-order optical nonlinear susceptibility. In this work, we present a convenient SHG technique to correct the false images by modulating laser polarization and stitching complementary images together, the stitched image will contain more detailed information and will better represent the sample structures. We first describe our SHG technique with a brief mathematical model, and then show its validity by performing it on two kind of samples: symmetrically synthesized bio-origin polymer poly(3-hyroxybutyrate-co-3- hydroxyhexanoate) (PHBHHx) and fibrillar collagen of rat-tail tendon.
AB - Second harmonic generation microscopy (SHGM) has been widely used in biomedicine as a promising non-invasive imaging technique. However, due to the complexity of biological samples, false images are often produced depending on sample orientation relative to laser polarization and on sample parameter related to the second-order optical nonlinear susceptibility. In this work, we present a convenient SHG technique to correct the false images by modulating laser polarization and stitching complementary images together, the stitched image will contain more detailed information and will better represent the sample structures. We first describe our SHG technique with a brief mathematical model, and then show its validity by performing it on two kind of samples: symmetrically synthesized bio-origin polymer poly(3-hyroxybutyrate-co-3- hydroxyhexanoate) (PHBHHx) and fibrillar collagen of rat-tail tendon.
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U2 - 10.1109/METAMAT.2006.335001
DO - 10.1109/METAMAT.2006.335001
M3 - Conference contribution
AN - SCOPUS:46149107089
SN - 0780397746
SN - 9780780397743
T3 - Proceedings of International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006
SP - 72
EP - 75
BT - Proceedings of International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006
PB - IEEE Computer Society
T2 - International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006
Y2 - 16 October 2006 through 18 October 2006
ER -