TY - JOUR
T1 - Tea Tree Oil Prevents Mastitis-Associated Inflammation in Lipopolysaccharide-Stimulated Bovine Mammary Epithelial Cells
AU - Chen, Zhi
AU - Zhang, Yi
AU - Zhou, Jingpeng
AU - Lu, Lu
AU - Wang, Xiaolong
AU - Liang, Yusheng
AU - Loor, Juan J.
AU - Gou, Deming
AU - Xu, Huifen
AU - Yang, Zhangping
N1 - Funding Information:
Funding. This research was supported by the National Natural Science Foundation of China (Grant Nos. 31802035, 31702100, 31872324, and 31601915), and the China Postdoctoral Science Foundation (Grant Nos. 2017M621841 and 2019T120472).
PY - 2020/8/7
Y1 - 2020/8/7
N2 - The main purpose of this study was to explore the effect of tea tree oil (TTO) on lipopolysaccharide (LPS)-induced mastitis model using isolated bovine mammary epithelial cells (BMEC). This mastitis model was used to determine cellular responses to TTO and LPS on cellular cytotoxicity, mRNA abundance and cytokine production. High-throughput sequencing was used to select candidate genes, followed by functional evaluation of those genes. In the first experiment, LPS at a concentration of 200 μg/mL reduced cell proliferation, induced apoptosis and upregulated protein concentrations of tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), and signal transducer and activator of transcription 1 (STAT1). Addition of TTO led to reduced cellular apoptosis along with downregulated protein concentrations of nuclear factor kappa B, mitogen-activated protein kinase 4 (MAPK4) and caspase-3. In the second experiment, BMEC challenged with LPS had a total of 1,270 differentially expressed genes of which 787 were upregulated and 483 were downregulated. Differentially expressed genes included TNF-α, IL6, STAT1, and MAPK4. Overall, results showed that TTO (at least in vitro) has a protective effect against LPS-induced mastitis. Further in vivo research should be performed to determine strategies for using TTO for prevention and treatment of mastitis and improvement of milk quality.
AB - The main purpose of this study was to explore the effect of tea tree oil (TTO) on lipopolysaccharide (LPS)-induced mastitis model using isolated bovine mammary epithelial cells (BMEC). This mastitis model was used to determine cellular responses to TTO and LPS on cellular cytotoxicity, mRNA abundance and cytokine production. High-throughput sequencing was used to select candidate genes, followed by functional evaluation of those genes. In the first experiment, LPS at a concentration of 200 μg/mL reduced cell proliferation, induced apoptosis and upregulated protein concentrations of tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), and signal transducer and activator of transcription 1 (STAT1). Addition of TTO led to reduced cellular apoptosis along with downregulated protein concentrations of nuclear factor kappa B, mitogen-activated protein kinase 4 (MAPK4) and caspase-3. In the second experiment, BMEC challenged with LPS had a total of 1,270 differentially expressed genes of which 787 were upregulated and 483 were downregulated. Differentially expressed genes included TNF-α, IL6, STAT1, and MAPK4. Overall, results showed that TTO (at least in vitro) has a protective effect against LPS-induced mastitis. Further in vivo research should be performed to determine strategies for using TTO for prevention and treatment of mastitis and improvement of milk quality.
KW - BMEC
KW - LPS
KW - TTO
KW - mastitis
KW - transcriptome sequencing
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U2 - 10.3389/fvets.2020.00496
DO - 10.3389/fvets.2020.00496
M3 - Article
C2 - 32851050
AN - SCOPUS:85089882661
VL - 7
JO - Frontiers in Veterinary Science
JF - Frontiers in Veterinary Science
SN - 2297-1769
M1 - 496
ER -