Targeting and expression of antigenic proteins in transgenic plants for production of edible oral vaccines

Research output: Contribution to journalReview articlepeer-review

Abstract

Exploiting plants as biological bioreactors for production and delivery of edible oral subunit vaccines is a promising application of biotechnology. Efforts to enhance expression levels of transgenes coding for antigenic proteins by exploiting promoters, targeting sequences, and enhancer elements have produced rather low quantities of the antigen in plant tissues, but enough to induce immune responses in feeding studies. This review will cover components of various gene constructs used in developing plant-based vaccines against a myriad of viral and bacterial diseases. Specifically, it will focus on sequences that are involved in targeting the antigen to mucosal tissues of the intestinal tract, thus enhancing the immunogenicity of the plant-based vaccine as well as those components that result in higher accumulation of the protein within the plant.

Original languageEnglish (US)
Pages (from-to)231-236
Number of pages6
JournalIn Vitro Cellular and Developmental Biology - Plant
Volume38
Issue number3
DOIs
StatePublished - 2002

Keywords

  • Biopharmaceuticals
  • Cholera toxin B
  • Escherichia coli
  • Heat-labile enterotoxin
  • Hepatitis B surface antigen
  • Molecular farming
  • Recombinant proteins
  • Respiratory syncytial virus

ASJC Scopus subject areas

  • Biotechnology
  • Plant Science

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