Targeted Ultrasound-Assisted Cancer-Selective Chemical Labeling and Subsequent Cancer Imaging using Click Chemistry

Hua Wang, Marianne Gauthier, Jamie R. Kelly, Rita J. Miller, Ming Xu, William D. O'Brien, Jianjun Cheng

Research output: Contribution to journalArticle

Abstract

Metabolic sugar labeling followed by the use of reagent-free click chemistry is an established technique for in vitro cell targeting. However, selective metabolic labeling of the target tissues in vivo remains a challenge to overcome, which has prohibited the use of this technique for targeted in vivo applications. Herein, we report the use of targeted ultrasound pulses to induce the release of tetraacetyl N-azidoacetylmannosamine (Ac4ManAz) from microbubbles (MBs) and its metabolic expression in the cancer area. Ac4ManAz-loaded MBs showed great stability under physiological conditions, but rapidly collapsed in the presence of tumor-localized ultrasound pulses. The released Ac4ManAz from MBs was able to label 4T1 tumor cells with azido groups and significantly improved the tumor accumulation of dibenzocyclooctyne (DBCO)-Cy5 by subsequent click chemistry. We demonstrated for the first time that Ac4ManAz-loaded MBs coupled with the use of targeted ultrasound could be a simple but powerful tool for in vivo cancer-selective labeling and targeted cancer therapies.

Original languageEnglish (US)
Pages (from-to)5452-5456
Number of pages5
JournalAngewandte Chemie - International Edition
Volume55
Issue number18
DOIs
StatePublished - Apr 25 2016

Keywords

  • cell labeling
  • click chemistry
  • drug design
  • sugars
  • ultrasound

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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