Targeted gene knockout by direct delivery of zinc-finger nuclease proteins

Thomas Gaj, Jing Guo, Yoshio Kato, Shannon J. Sirk, Carlos F. Barbas

Research output: Contribution to journalArticlepeer-review


Zinc-finger nucleases (ZFNs) are versatile reagents that have redefined genome engineering. Realizing the full potential of this technology requires the development of safe and effective methods for delivering ZFNs into cells. We demonstrate the intrinsic cell-penetrating capabilities of the standard ZFN architecture and show that direct delivery of ZFNs as proteins leads to efficient endogenous gene disruption in various mammalian cell types with minimal off-target effects.

Original languageEnglish (US)
Pages (from-to)805-807
Number of pages3
JournalNature Methods
Issue number8
StatePublished - Aug 2012

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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