Target molecular size suggesting a dimeric structure for the red beet plasma membrane Ca2+-ATPase

Swati Basu, Donald P. Briskin

Research output: Contribution to journalArticle

Abstract

The target molecular size of the red beet (Beta vulgaris L. var. Detroit Dark Red) plasma membrane Ca2+-ATPase was determined by radiation inactivation analysis involving γ-rays from a 60Co source. The Ca2+ transport activity associated with the Ca2+-ATPase in native plasma membrane vesicles declined in an exponential manner with radiation dose and the target molecular size was estimated to be about 245 000 ± 5000. As the catalytic subunit (phosphorylated intermediate) of the red beet plasma membrane Ca2+-ATPase has been shown to be about 100 000 to 120 000, these results suggest that the enzyme exists as a dimer in the native membrane.

Original languageEnglish (US)
Pages (from-to)15-17
Number of pages3
JournalPhytochemistry
Volume38
Issue number1
DOIs
StatePublished - Jan 1995

Keywords

  • Beta vulgaris
  • Chenopodiaceae
  • beet
  • membrane transport
  • radiation inactivation analysis
  • signal transduction
  • transport ATPase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Plant Science
  • Horticulture

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