TY - JOUR
T1 - Taranis Protects Regenerating Tissue from Fate Changes Induced by the Wound Response in Drosophila
AU - Schuster, Keaton J.
AU - Smith-Bolton, Rachel K.
N1 - Funding Information:
We thank A.R. Brock, S.J. Khan, S. Siegrist, L. Buttita, and W.M. Brieher for critical reading of the manuscript. We thank the Bloomington Drosophila Stock Center, the Flytrap Project, the Developmental Studies Hybridoma Bank, S. Siegrist, S. Cohen, and M. Cleary for reagents. We thank Sha Peng for technical assistance with qRT-PCR. This work was supported by a Roy J. Carver Charitable Trust Young Investigator Award (12-4041).
Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2015/7/6
Y1 - 2015/7/6
N2 - Regenerating tissue must replace lost structures with cells of the proper identity and function. How regenerating tissue establishes or maintains correct cell fates during regrowth is an open question. We have identified a gene, taranis, that is essential for maintaining proper cell fate in damaged and regenerating Drosophila wing imaginal discs but that is dispensable for these fates in normal wing development. In regenerating tissue with reduced levels of Taranis, expression of the posterior selector gene engrailed is silenced through an autoregulatory silencing mechanism that requires the PRC1 component polyhomeotic, resulting in a transformation of posterior tissue into anterior tissue late in regeneration. An essential component of the wound response, JNK signaling, induces this misregulation of engrailed expression. Taranis can suppress these JNK-induced cell fate changes without interfering with JNK signaling activity. Thus, taranis protects regenerating tissue from deleterious side effects of wound healing and regeneration.
AB - Regenerating tissue must replace lost structures with cells of the proper identity and function. How regenerating tissue establishes or maintains correct cell fates during regrowth is an open question. We have identified a gene, taranis, that is essential for maintaining proper cell fate in damaged and regenerating Drosophila wing imaginal discs but that is dispensable for these fates in normal wing development. In regenerating tissue with reduced levels of Taranis, expression of the posterior selector gene engrailed is silenced through an autoregulatory silencing mechanism that requires the PRC1 component polyhomeotic, resulting in a transformation of posterior tissue into anterior tissue late in regeneration. An essential component of the wound response, JNK signaling, induces this misregulation of engrailed expression. Taranis can suppress these JNK-induced cell fate changes without interfering with JNK signaling activity. Thus, taranis protects regenerating tissue from deleterious side effects of wound healing and regeneration.
UR - http://www.scopus.com/inward/record.url?scp=84942417392&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84942417392&partnerID=8YFLogxK
U2 - 10.1016/j.devcel.2015.04.017
DO - 10.1016/j.devcel.2015.04.017
M3 - Article
C2 - 26096735
AN - SCOPUS:84942417392
SN - 1534-5807
VL - 34
SP - 119
EP - 128
JO - Developmental Cell
JF - Developmental Cell
IS - 1
ER -