Tailoring gelation and mechanical properties of fibrin-based extracellular matrix using stimulus-responsive poly(Lactic-co-glycolic acid) microgelator

Yu Tong Hong, Hyun Joon Kong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Statement of Purpose: Fibrin based matrices have been extensively used for three dimensional cell culture, tissue regeneration, and injectable cellular carrier due to their ability to support cell activities responsible for tissue regeneration 1 . However, there are still grand needs to control the gelation rate and the mechanical strength for enhanced transports and therapeutic efficacy of cells. This challenge results from a difficulty in regulating the gelation time while maintaining the mechanical properties and microstructure of fibrin matrices due to the complex protein polymerization mechanism 2 . To this end, this study demonstrates the stimulus-responsive poly (lactic-co-glycolic acid) (PLGA) microgelator that can activate fibrin fiber formation from their surfaces by actively ejecting thrombin in response to an external stimulus (i.e., hydrogen peroxide). The stimulus-responsive microgelator was assembled by simultaneously encapsulating MnO 2 nanosheets that decompose H 2 O 2 to O 2 gas and thrombin into a spherical shell. We hypothesize that the microgelator would generates oxygen within the particles when exposed to H 2 O 2 . The oxygen gas will then act as force to pump out thrombin from the particles by increasing the internal pressure of particles. In the fibrinogen solution, the ejected thrombin molecules nucleate the fibrin fibers from the PLGA particle surface and finally form the interconnected fibrous networks with an increased elastic modulus, compared to the fibrin gel formed by mixing fibrinogen and thrombin in solution.

Original languageEnglish (US)
Title of host publicationSociety for Biomaterials Annual Meeting and Exposition 2019
Subtitle of host publicationThe Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting
PublisherSociety for Biomaterials
Number of pages1
ISBN (Electronic)9781510883901
StatePublished - Jan 1 2019
Event42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Seattle, United States
Duration: Apr 3 2019Apr 6 2019

Publication series

NameTransactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
Volume40
ISSN (Print)1526-7547

Conference

Conference42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
CountryUnited States
CitySeattle
Period4/3/194/6/19

Fingerprint

Tissue regeneration
Gelation
Fibrin
Thrombin
Extracellular Matrix
Mechanical properties
Tissue culture
Oxygen
Acids
Fibers
Nanosheets
Gases
Hydrogen peroxide
Strength of materials
Gels
Elastic moduli
Fibrinogen
Polymerization
Pumps
Regeneration

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Biotechnology
  • Biomaterials
  • Materials Chemistry

Cite this

Hong, Y. T., & Kong, H. J. (2019). Tailoring gelation and mechanical properties of fibrin-based extracellular matrix using stimulus-responsive poly(Lactic-co-glycolic acid) microgelator. In Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium; Vol. 40). Society for Biomaterials.

Tailoring gelation and mechanical properties of fibrin-based extracellular matrix using stimulus-responsive poly(Lactic-co-glycolic acid) microgelator. / Hong, Yu Tong; Kong, Hyun Joon.

Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Society for Biomaterials, 2019. (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium; Vol. 40).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hong, YT & Kong, HJ 2019, Tailoring gelation and mechanical properties of fibrin-based extracellular matrix using stimulus-responsive poly(Lactic-co-glycolic acid) microgelator. in Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium, vol. 40, Society for Biomaterials, 42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence, Seattle, United States, 4/3/19.
Hong YT, Kong HJ. Tailoring gelation and mechanical properties of fibrin-based extracellular matrix using stimulus-responsive poly(Lactic-co-glycolic acid) microgelator. In Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Society for Biomaterials. 2019. (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium).
Hong, Yu Tong ; Kong, Hyun Joon. / Tailoring gelation and mechanical properties of fibrin-based extracellular matrix using stimulus-responsive poly(Lactic-co-glycolic acid) microgelator. Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Society for Biomaterials, 2019. (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium).
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