Abstract

Serotonin (5-hydroxytryptamine, 5-HT) is a ubiquitous modulatory neurotransmitter with roles as a neurohormone and neurotransmitter. However, few studies have been performed characterizing this molecule and its related metabolites in circulating fluids. Here, we demonstrate native 5-HT sulfate, but much lower levels of 5-HT, in hemolymph of the marine mollusk Pleurobranchaea californica. The metabolite 5-HT sulfate forms from 5-HT uptake and metabolism in central ganglia of Aplysia californica and in the visceral nerve and eye of Pleurobranchaea, but not in hemolymph itself. In addition, 5-hydroxyindole acetic acid (5-HIAA), while not detected in hemolymph, forms in higher quantities than does 5-HT sulfate in the eye and visceral nerve, and γ-glu-5-HT is also observed in this area but never in hemolymph. As systemic 5-HT sulfate appears not to originate from the optic region or from systemic 5-HT, 5-HT sulfate likely derives from the nervous system. Circulating 5-HT sulfate is at least 10-fold higher during the light portion of a 12: 12-h light/dark cycle than during the dark portion (p < 0.0007), but there is no obvious trend for free systemic tryptophan (Trp) (p > 0.3) in Pleurobranchaea. 5-HT in mollusks is associated with general arousal state; thus, diurnal systemic changes in a 5-HT catabolite may reflect a regulatory role for indole catabolism in behavioral rhythms.

Original languageEnglish (US)
Pages (from-to)734-742
Number of pages9
JournalJournal of Neurochemistry
Volume90
Issue number3
DOIs
StatePublished - Aug 1 2004

Fingerprint

Mollusca
Serotonin
Sulfates
Pleurobranchaea
Hemolymph
Neurotransmitter Agents
Metabolites
5-hydroxytryptamine O-sulfate
Aplysia
Photoperiod
Neurology
Arousal
Metabolism
Ganglia
Acetic Acid
Nervous System
Optics

Keywords

  • Capillary electrophoresis
  • Hemolymph
  • Marine mollusk
  • Serotonin
  • Sulfation

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Systemic serotonin sulfate in opisthobranch mollusks. / Stuart, Jeffrey N.; Ebaugh, Jason D.; Copes, Ashley L.; Hatcher, Nathan G.; Gillette, Rhanor; Sweedler, Jonathan V.

In: Journal of Neurochemistry, Vol. 90, No. 3, 01.08.2004, p. 734-742.

Research output: Contribution to journalArticle

Stuart, Jeffrey N. ; Ebaugh, Jason D. ; Copes, Ashley L. ; Hatcher, Nathan G. ; Gillette, Rhanor ; Sweedler, Jonathan V. / Systemic serotonin sulfate in opisthobranch mollusks. In: Journal of Neurochemistry. 2004 ; Vol. 90, No. 3. pp. 734-742.
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