System analysis of Lipomyces starkeyi during growth on various plant-based sugars

Anshu Deewan, Jing Jing Liu, Sujit Sadashiv Jagtap, Eun Ju Yun, Hanna Walukiewicz, Yong Su Jin, Christopher V. Rao

Research output: Contribution to journalArticlepeer-review

Abstract

Abstract: Oleaginous yeasts have received significant attention due to their substantial lipid storage capability. The accumulated lipids can be utilized directly or processed into various bioproducts and biofuels. Lipomyces starkeyi is an oleaginous yeast capable of using multiple plant-based sugars, such as glucose, xylose, and cellobiose. It is, however, a relatively unexplored yeast due to limited knowledge about its physiology. In this study, we have evaluated the growth of L. starkeyi on different sugars and performed transcriptomic and metabolomic analyses to understand the underlying mechanisms of sugar metabolism. Principal component analysis showed clear differences resulting from growth on different sugars. We have further reported various metabolic pathways activated during growth on these sugars. We also observed non-specific regulation in L. starkeyi and have updated the gene annotations for the NRRL Y-11557 strain. This analysis provides a foundation for understanding the metabolism of these plant-based sugars and potentially valuable information to guide the metabolic engineering of L. starkeyi to produce bioproducts and biofuels. Key points: • L. starkeyi metabolism reprograms for consumption of different plant-based sugars. • Non-specific regulation was observed during growth on cellobiose. • L. starkeyi secretes β-glucosidases for extracellular hydrolysis of cellobiose.

Original languageEnglish (US)
Pages (from-to)5629-5642
Number of pages14
JournalApplied Microbiology and Biotechnology
Volume106
Issue number17
DOIs
StatePublished - Sep 2022

Keywords

  • Anshu Deewan and Jing-Jing Liu contributed equally to this work
  • Lipomyces starkeyi
  • Metabolomics
  • Oleaginous yeast
  • RNA sequencing
  • Sugar uptake
  • Transcriptomics

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biotechnology

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