TY - JOUR
T1 - Synthetic Macrocycle Nanopore for Potassium-Selective Transmembrane Transport
AU - Qiao, Dan
AU - Joshi, Himanshu
AU - Zhu, Huangtianzhi
AU - Wang, Fushi
AU - Xu, Yang
AU - Gao, Jia
AU - Huang, Feihe
AU - Aksimentiev, Aleksei
AU - Feng, Jiandong
N1 - Funding Information:
This study was funded by the Natural Science Foundation of Zhejiang Province (LR20B050002), the National Natural Science Foundation of China (21974123), the National Key R&D Program of China (2020YFA0211200), the Fundamental Research Funds for the Central Universities, China (2019XZZX003-01), and the Hundreds Program of Zhejiang University. A.A. and H.J. acknowledge support from the National Science Foundation of USA (DMR-1827346) and the supercomputer time provided through the XSEDE allocation grant (MCA05S028) and the Leadership Resource Allocation MCB20012 on Frontera of the Texas Advanced Computing Center. We also thank the Center of Analytical Service at Zhejiang University for help with MALDI-TOF measurements and the Cryo-EM Center at Zhejiang University for access to TEM.
Publisher Copyright:
© 2021 American Chemical Society.
PY - 2021/10/6
Y1 - 2021/10/6
N2 - Reproducing the structure and function of biological membrane channels, synthetic nanopores have been developed for applications in membrane filtration technologies and biomolecular sensing. Stable stand-alone synthetic nanopores have been created from a variety of materials, including peptides, nucleic acids, synthetic polymers, and solid-state membranes. In contrast to biological nanopores, however, furnishing such synthetic nanopores with an atomically defined shape, including deliberate placement of each and every chemical group, remains a major challenge. Here, we introduce a chemosynthetic macromolecule-extended pillararene macrocycle (EPM)-as a chemically defined transmembrane nanopore that exhibits selective transmembrane transport. Our ionic current measurements reveal stable insertion of individual EPM nanopores into a lipid bilayer membrane and remarkable cation type-selective transport, with up to a 21-fold selectivity for potassium over sodium ions. Taken together, direct chemical synthesis offers a path to de novo design of a new class of synthetic nanopores with custom transport functionality imprinted in their atomically defined chemical structure.
AB - Reproducing the structure and function of biological membrane channels, synthetic nanopores have been developed for applications in membrane filtration technologies and biomolecular sensing. Stable stand-alone synthetic nanopores have been created from a variety of materials, including peptides, nucleic acids, synthetic polymers, and solid-state membranes. In contrast to biological nanopores, however, furnishing such synthetic nanopores with an atomically defined shape, including deliberate placement of each and every chemical group, remains a major challenge. Here, we introduce a chemosynthetic macromolecule-extended pillararene macrocycle (EPM)-as a chemically defined transmembrane nanopore that exhibits selective transmembrane transport. Our ionic current measurements reveal stable insertion of individual EPM nanopores into a lipid bilayer membrane and remarkable cation type-selective transport, with up to a 21-fold selectivity for potassium over sodium ions. Taken together, direct chemical synthesis offers a path to de novo design of a new class of synthetic nanopores with custom transport functionality imprinted in their atomically defined chemical structure.
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U2 - 10.1021/jacs.1c04910
DO - 10.1021/jacs.1c04910
M3 - Article
C2 - 34403582
SN - 0002-7863
VL - 143
SP - 15975
EP - 15983
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 39
ER -