Synthetic holographic phase imaging in confocal microscopy and applications

M. Schnell, S. Buercklin, P. Sarriugarte, M. J. Perez-Roldan, R. Hillenbrand, P. S. Carney

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present synthetic optical holography (SOH) as a new phase imaging modality in confocal microscopy, where amplitude and phase information are encoded holographically by a reference wave with a linear-in-time phase function. The implementation of SOH is technically simple, only requiring the addition of a linearly moving reference mirror. We demonstrate reliable confocal optical topography mapping with sub-nanometer depth resolution. Furthermore, we show numerical refocusing of out-of-focus confocal data, restoring a spatial resolution near the diffraction limit for situations where the sample is located up to 60 microns away from the system's focal plane. We envision that quantitative phase imaging as provided by SOH will allow for a host of new possibilities in monochromatic confocal microscopy, ranging from stain- and dye-free confocal bio-imaging and phase-resolved second harmonic microscopy to threedimensional imaging and computational aberration correction.

Original languageEnglish (US)
Title of host publicationFrontiers in Optics, FiO 2016
PublisherOSA - The Optical Society
ISBN (Print)9781943580194
DOIs
StatePublished - Jul 21 2014
Externally publishedYes
EventFrontiers in Optics, FiO 2016 - Rochester, United States
Duration: Oct 17 2016Oct 21 2016

Publication series

NameOptics InfoBase Conference Papers

Conference

ConferenceFrontiers in Optics, FiO 2016
Country/TerritoryUnited States
CityRochester
Period10/17/1610/21/16

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Mechanics of Materials

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