TY - JOUR
T1 - Synthesis of an O-glycosylated cell surface protein induced in yeast by α factor
AU - Orlean, P.
AU - Ammer, H.
AU - Watzele, M.
AU - Tanner, W.
PY - 1986
Y1 - 1986
N2 - A number of cell surface glycoproteins can be specifically and completely released from intact cells of Saccharomyces cerevisiae with 0.5% mercaptoethanol. Among these proteins is one with a molecular mass of 22 kDa, which is synthesized only in haploid a cells treated with the peptide mating pheromone α factor. This protein could be radiolabeled in vivo with [2-3H]mannose, [14C]phenylaline, and [35S]sulfate. Its synthesis and export to the cell surface were not inhibited by tunicamycin. β-Elimination released almost all radioactivity from the [2-3H]mannose-labeled protein, 36% of its radioactivity being recovered subsequently as mannose and 43% as a dimannoside. Evidence is presented that the 22-kDa O-glycosylated protein is a mating-specific a cell agglutinin.
AB - A number of cell surface glycoproteins can be specifically and completely released from intact cells of Saccharomyces cerevisiae with 0.5% mercaptoethanol. Among these proteins is one with a molecular mass of 22 kDa, which is synthesized only in haploid a cells treated with the peptide mating pheromone α factor. This protein could be radiolabeled in vivo with [2-3H]mannose, [14C]phenylaline, and [35S]sulfate. Its synthesis and export to the cell surface were not inhibited by tunicamycin. β-Elimination released almost all radioactivity from the [2-3H]mannose-labeled protein, 36% of its radioactivity being recovered subsequently as mannose and 43% as a dimannoside. Evidence is presented that the 22-kDa O-glycosylated protein is a mating-specific a cell agglutinin.
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U2 - 10.1073/pnas.83.17.6263
DO - 10.1073/pnas.83.17.6263
M3 - Article
C2 - 16593749
AN - SCOPUS:0023009333
SN - 0027-8424
VL - 83
SP - 6263
EP - 6266
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 17
ER -