Synthesis and characterisation of N-gene targeted NIR-II fluorescent probe for selective localisation of SARS-CoV-2

Parikshit Moitra; Maha Alafeef; Ketan Dighe; Zach Sheffield; Dipendra Dahal; Dipanjan Pan

doi:10.1039/d1cc01410b

Synthesis and characterisation of N-gene targeted NIR-II fluorescent probe for selective localisation of SARS-CoV-2

Parikshit Moitra, Maha Alafeef, Ketan Dighe, Zach Sheffield, Dipendra Dahal, Dipanjan Pan

Bioengineering

Research output: Contribution to journal › Article › peer-review

Abstract

Tracking the viral progression of SARS-CoV-2 in COVID-19 infected body tissues is an emerging need of the current pandemic. Imaging at near infrared second biological window (NIR-II) offers striking benefits over the other technologies to explore deep-tissue information. Here we design, synthesise and characterise a molecular probe that selectively targets the N-gene of SARS-CoV-2. Highly specific antisense oligonucleotides (ASOs) were conjugated to lead sulfide quantum dots using a UV-triggered thiol-ene click chemistry for the recognition of viral RNA. Our ex vivo imaging studies demonstrated that the probe exhibits aggregation induced NIR-II emission only in presence of SARS-CoV-2 RNA which can be attributed to the efficient hybridisation of the ASOs with their target RNA strands.

Original language	English (US)
Pages (from-to)	6229-6232
Number of pages	4
Journal	Chemical Communications
Volume	57
Issue number	51
Early online date	May 28 2021
DOIs	https://doi.org/10.1039/d1cc01410b
State	Published - Jun 28 2021

Keywords

COVID-19
severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Pandemic

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Ceramics and Composites
Metals and Alloys
Materials Chemistry
Surfaces, Coatings and Films
Chemistry(all)
Catalysis

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10.1039/d1cc01410b

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AU - Moitra, Parikshit

AU - Alafeef, Maha

AU - Dighe, Ketan

AU - Sheffield, Zach

AU - Dahal, Dipendra

AU - Pan, Dipanjan

PY - 2021/6/28

Y1 - 2021/6/28

N2 - Tracking the viral progression of SARS-CoV-2 in COVID-19 infected body tissues is an emerging need of the current pandemic. Imaging at near infrared second biological window (NIR-II) offers striking benefits over the other technologies to explore deep-tissue information. Here we design, synthesise and characterise a molecular probe that selectively targets the N-gene of SARS-CoV-2. Highly specific antisense oligonucleotides (ASOs) were conjugated to lead sulfide quantum dots using a UV-triggered thiol-ene click chemistry for the recognition of viral RNA. Our ex vivo imaging studies demonstrated that the probe exhibits aggregation induced NIR-II emission only in presence of SARS-CoV-2 RNA which can be attributed to the efficient hybridisation of the ASOs with their target RNA strands.

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Synthesis and characterisation of N-gene targeted NIR-II fluorescent probe for selective localisation of SARS-CoV-2

Abstract

Keywords

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