Synergy between nutritional immunity and independent host defenses contributes to the importance of the MntABC manganese transporter during staphylococcus aureus infection

Jana N. Radin, Jamie Zhu, Erin B. Brazel, Christopher A. McDevitt, Thomas E. Kehl-Fiea

Research output: Contribution to journalArticle

Abstract

During infection, the host utilizes a diverse array of processes to combat invaders, including the restriction of availability of essential nutrients such as manganese. Similarly to many other pathogens, Staphylococcus aureus possesses two manganese importers, MntH and MntABC. Several infection models have revealed a critical role for MntABC during staphylococcal infection. However, culture-based studies have suggested parity between the two transporters when cells are resisting manganese starvation imposed by the manganese binding immune effector calprotectin. In this investigation, initial elemental analysis revealed that MntABC is the primary transporter responsible for obtaining manganese in culture in the presence of calprotectin. MntABC was also necessary to maintain wild-type levels of manganese-dependent superoxide dismutase activity in the presence of calprotectin. Building on this framework, we investigated if MntABC enabled S. aureus to resist the synergistic actions of nutritional immunity and other host defenses. This analysis revealed that MntABC critically contributes to staphylococcal growth when S. aureus is subjected to manganese limitations and exposed to oxidative stress. This transporter was also important for growth in manganese-limited environments when S. aureus was forced to consume glucose as an energy source, which occurs when it encounters nitric oxide. MntABC also expanded the pH range conducive for S. aureus growth under conditions of manganese scarcity. Collectively, the data presented in this work provide a robust molecular basis for the crucial role of MntABC in staphylococcal virulence. Further, this work highlights the importance of synergy between host defenses and the necessity of evaluating the contribution of virulence factors to pathogenesis in the presence of multiple stressors.

Original languageEnglish (US)
Article numbere00642
JournalInfection and immunity
Volume87
Issue number1
DOIs
StatePublished - Jan 1 2019

Fingerprint

Manganese
Staphylococcus aureus
Immunity
Leukocyte L1 Antigen Complex
Infection
Growth
Staphylococcal Infections
Virulence Factors
Starvation
Parity
Superoxide Dismutase
Virulence
Nitric Oxide
Oxidative Stress
Glucose
Food

Keywords

  • ABC transporters
  • Calprotectin
  • Infection
  • Manganese
  • MntABC
  • MntH
  • Nutritional immunity
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Synergy between nutritional immunity and independent host defenses contributes to the importance of the MntABC manganese transporter during staphylococcus aureus infection. / Radin, Jana N.; Zhu, Jamie; Brazel, Erin B.; McDevitt, Christopher A.; Kehl-Fiea, Thomas E.

In: Infection and immunity, Vol. 87, No. 1, e00642, 01.01.2019.

Research output: Contribution to journalArticle

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