Synergistic binding of the leader and core peptides by the lantibiotic synthetase HalM2

Gabrielle N. Thibodeaux, Amanda L. McClerren, Yunli Ma, Marc R. Gancayco, Wilfred A. Van Der Donk

Research output: Contribution to journalArticlepeer-review


Lanthipeptides are a class of ribosomally produced and post-translationally modified peptides (RiPPs) that possess a variety of biological activities but typically act as antimicrobial agents (lantibiotics). Haloduracin is a lantibiotic that is composed of two post-translationally modified peptides, Halα and Halβ, which are biosynthesized from the precursor peptides HalA1 and HalA2 by their cognate lanthipeptide synthetases, HalM1 and HalM2, respectively. Coexpression studies of HalM1 and HalM2 with chimeric peptides consisting of the leader peptide of HalA1 and the core peptide of HalA2 (or vice versa) showed that the synthetases require both the cognate leader and core peptides for efficient processing. Investigation of the affinity in vitro showed that binding of the N-terminal leader peptide by HalM2 increases its affinity for the C-terminal core peptide. Thus, the two segments of the precursor peptide HalA2 synergistically bind to HalM2.

Original languageEnglish (US)
Pages (from-to)970-977
Number of pages8
JournalACS chemical biology
Issue number4
StatePublished - Apr 17 2015

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine


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