Surface tethering of stromal cell-derived factor-1α carriers to stem cells enhances cell homing to ischemic muscle

Jye Yng Teo, Eunkyung Ko, Jiayu Leong, Jiman Hong, Jessie S. Jeon, Yi Yan Yang, Hyunjoon Kong

Research output: Contribution to journalArticlepeer-review

Abstract

Mesenchymal stem cells are promising medicine for treating diseases and tissue defects because of their innate ability to secrete therapeutic factors. Intravenous delivery of stem cells, although favored for its minimal invasiveness, is often plagued by low cellular engraftment in the target tissue. To this end, this study hypothesizes that in situ activation of cellular expression of CXC chemokine 4 (CXCR4) would significantly improve cellular migration to injured tissue. This hypothesis was examined by tethering the surface of stem cells with poly(D,L-lactide-co-glycolide)-block-hyaluronic acid (HA) particles containing stromal cell-derived factor-1α, a model chemokine to sensitize CXCR4. The HA blocks in the particles enhanced the association rate constant to stem cells by 3.3-fold, and in turn, increased the number of cells expressing CXCR4 receptors. Consequently, these cells displayed 1.2-fold higher transendothelial migration in vitro and 1.7-fold greater trafficking to the ischemic hindlimb of a mouse than that of the untethered cells.

Original languageEnglish (US)
Article number102215
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume28
DOIs
StatePublished - Aug 2020

Keywords

  • CXC chemokine 4
  • Ischemia
  • Mesenchymal stem cells
  • Particles
  • Stromal cell-derived factor-1α

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

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