@article{9a76024339c142f2bba492bcf74b68ad,
title = "Surface tethering of stromal cell-derived factor-1α carriers to stem cells enhances cell homing to ischemic muscle",
abstract = "Mesenchymal stem cells are promising medicine for treating diseases and tissue defects because of their innate ability to secrete therapeutic factors. Intravenous delivery of stem cells, although favored for its minimal invasiveness, is often plagued by low cellular engraftment in the target tissue. To this end, this study hypothesizes that in situ activation of cellular expression of CXC chemokine 4 (CXCR4) would significantly improve cellular migration to injured tissue. This hypothesis was examined by tethering the surface of stem cells with poly(D,L-lactide-co-glycolide)-block-hyaluronic acid (HA) particles containing stromal cell-derived factor-1α, a model chemokine to sensitize CXCR4. The HA blocks in the particles enhanced the association rate constant to stem cells by 3.3-fold, and in turn, increased the number of cells expressing CXCR4 receptors. Consequently, these cells displayed 1.2-fold higher transendothelial migration in vitro and 1.7-fold greater trafficking to the ischemic hindlimb of a mouse than that of the untethered cells.",
keywords = "CXC chemokine 4, Ischemia, Mesenchymal stem cells, Particles, Stromal cell-derived factor-1α",
author = "Teo, {Jye Yng} and Eunkyung Ko and Jiayu Leong and Jiman Hong and Jeon, {Jessie S.} and Yang, {Yi Yan} and Hyunjoon Kong",
note = "Funding Information: We thank M. J. Dailey and S. Chaki from the Department of Animal Sciences for their guidance in using the tissue homogenizer. This work was supported by the National Institutes of Health, USA (Grant 1R01 HL109192 and 1R21 HL131469 to H. Kong) and National Science Foundation, USA (CBET-1403491 to H. Kong). This work was also supported by the National Institute of Biomedical Imaging and Bioengineering of the National Institutes of Health, USA (Award T32EB019944 to E. Ko). J.Y. Teo and J. Leong acknowledge the support of A*STAR Graduate Scholarship (Overseas) from the Agency for Science, Technology, and Research (A*STAR), Singapore. Y.Y. Yang acknowledges the support from the Institute of Bioengineering and Nanotechnology, A*STAR, Singapore. Funding Information: We thank M. J. Dailey and S. Chaki from the Department of Animal Sciences for their guidance in using the tissue homogenizer. This work was supported by the National Institutes of Health , USA (Grant 1R01 HL109192 and 1R21 HL131469 to H. Kong) and National Science Foundation , USA ( CBET-1403491 to H. Kong). This work was also supported by the National Institute of Biomedical Imaging and Bioengineering of the National Institutes of Health , USA (Award T32EB019944 to E. Ko). J.Y. Teo and J. Leong acknowledge the support of A*STAR Graduate Scholarship (Overseas) from the Agency for Science, Technology, and Research (A*STAR), Singapore. Y.Y. Yang acknowledges the support from the Institute of Bioengineering and Nanotechnology, A*STAR, Singapore. Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2020",
month = aug,
doi = "10.1016/j.nano.2020.102215",
language = "English (US)",
volume = "28",
journal = "Nanomedicine: Nanotechnology, Biology, and Medicine",
issn = "1549-9634",
publisher = "Elsevier Inc.",
}