Surface tethering of stem cells with H2O2-responsive anti-oxidizing colloidal particles for protection against oxidation-induced death

Jye Yng Teo; Yongbeom Seo; Eunkyung Ko; Jiayu Leong; Yu Tong Hong; Yi Yan Yang; Hyunjoon Kong

doi:10.1016/j.biomaterials.2019.01.039

Surface tethering of stem cells with H₂O₂-responsive anti-oxidizing colloidal particles for protection against oxidation-induced death

Jye Yng Teo, Yongbeom Seo, Eunkyung Ko, Jiayu Leong, Yu Tong Hong, Yi Yan Yang, Hyunjoon Kong

Research output: Contribution to journal › Article › peer-review

Abstract

Mesenchymal stem cells are the new generation of medicine for treating numerous vascular diseases and tissue defects because of their ability to secrete therapeutic factors. Poor cellular survival in an oxidative diseased tissue, however, hinders the therapeutic efficacy. To this end, we hypothesized that tethering the surface of stem cells with colloidal particles capable of discharging antioxidant cargos in response to elevated levels of hydrogen peroxide (H ₂ O ₂ ) would maintain survival and therapeutic activity of the stem cells. We examined this hypothesis by encapsulating epigallocatechin gallate (EGCG) and manganese oxide (MnO ₂ ) nanocatalysts into particles comprising poly(D,L-lactide-co-glycolide)-block-hyaluronic acid. The MnO ₂ nanocatalysts catalyzed the decomposition of H ₂ O ₂ into oxygen gas, which increased the internal pressure of particles and accelerated the release of EGCG by 1.5-fold. Consequently, stem cells exhibited 1.2-fold higher metabolic activity and 2.8-fold higher secretion level of pro-angiogenic factor in sub-lethal H ₂ O ₂ concentrations. These stem cells, in turn, performed a greater angiogenic potential with doubled number of newly formed mature blood vessels. We envisage that the results of this study will contribute to improving the therapeutic efficacy of a wide array of stem cells.

Original language	English (US)
Pages (from-to)	1-15
Number of pages	15
Journal	Biomaterials
Volume	201
DOIs	https://doi.org/10.1016/j.biomaterials.2019.01.039
State	Published - May 2019

Keywords

Colloidal particles
Epigallocatechin gallate
Hydrogen peroxide
Manganese oxide nanocatalysts
Mesenchymal stem cells
Surface tethering

ASJC Scopus subject areas

Bioengineering
Ceramics and Composites
Biophysics
Biomaterials
Mechanics of Materials

Online availability

10.1016/j.biomaterials.2019.01.039

Library availability

Discover UIUC Full Text

Cite this

@article{9033599a057c4674ac4f3e102d6e1548,

title = "Surface tethering of stem cells with H2O2-responsive anti-oxidizing colloidal particles for protection against oxidation-induced death",

abstract = " Mesenchymal stem cells are the new generation of medicine for treating numerous vascular diseases and tissue defects because of their ability to secrete therapeutic factors. Poor cellular survival in an oxidative diseased tissue, however, hinders the therapeutic efficacy. To this end, we hypothesized that tethering the surface of stem cells with colloidal particles capable of discharging antioxidant cargos in response to elevated levels of hydrogen peroxide (H 2 O 2 ) would maintain survival and therapeutic activity of the stem cells. We examined this hypothesis by encapsulating epigallocatechin gallate (EGCG) and manganese oxide (MnO 2 ) nanocatalysts into particles comprising poly(D,L-lactide-co-glycolide)-block-hyaluronic acid. The MnO 2 nanocatalysts catalyzed the decomposition of H 2 O 2 into oxygen gas, which increased the internal pressure of particles and accelerated the release of EGCG by 1.5-fold. Consequently, stem cells exhibited 1.2-fold higher metabolic activity and 2.8-fold higher secretion level of pro-angiogenic factor in sub-lethal H 2 O 2 concentrations. These stem cells, in turn, performed a greater angiogenic potential with doubled number of newly formed mature blood vessels. We envisage that the results of this study will contribute to improving the therapeutic efficacy of a wide array of stem cells.",

keywords = "Colloidal particles, Epigallocatechin gallate, Hydrogen peroxide, Manganese oxide nanocatalysts, Mesenchymal stem cells, Surface tethering",

author = "Teo, {Jye Yng} and Yongbeom Seo and Eunkyung Ko and Jiayu Leong and Hong, {Yu Tong} and Yang, {Yi Yan} and Hyunjoon Kong",

note = "Funding Information: This work was supported by the National Institutes of Health (Grant 1R01 HL109192 and 1 R21 HL131469 to H. Kong) and National Science Foundation ( CBET-1403491 to H. Kong). J.Y. Teo and J. Leong acknowledge the support of A*STAR Graduate Scholarship (Overseas) from the Agency for Science, Technology, and Research (A*STAR), Singapore . E. Ko acknowledges the support from the National Institute of Biomedical Imaging and Bioengineering of the National Institutes of Health (Award T32EB019944 ). Y.Y. Yang acknowledges the support from the Institute of Bioengineering and Nanotechnology (Biomedical Research Council, Agency for Science, Technology, and Research), Singapore . Appendix A Publisher Copyright: {\textcopyright} 2019",

year = "2019",

month = may,

doi = "10.1016/j.biomaterials.2019.01.039",

language = "English (US)",

volume = "201",

pages = "1--15",

journal = "Biomaterials",

issn = "0142-9612",

publisher = "Elsevier BV",

}

TY - JOUR

T1 - Surface tethering of stem cells with H2O2-responsive anti-oxidizing colloidal particles for protection against oxidation-induced death

AU - Teo, Jye Yng

AU - Seo, Yongbeom

AU - Ko, Eunkyung

AU - Leong, Jiayu

AU - Hong, Yu Tong

AU - Yang, Yi Yan

AU - Kong, Hyunjoon

N1 - Funding Information: This work was supported by the National Institutes of Health (Grant 1R01 HL109192 and 1 R21 HL131469 to H. Kong) and National Science Foundation ( CBET-1403491 to H. Kong). J.Y. Teo and J. Leong acknowledge the support of A*STAR Graduate Scholarship (Overseas) from the Agency for Science, Technology, and Research (A*STAR), Singapore . E. Ko acknowledges the support from the National Institute of Biomedical Imaging and Bioengineering of the National Institutes of Health (Award T32EB019944 ). Y.Y. Yang acknowledges the support from the Institute of Bioengineering and Nanotechnology (Biomedical Research Council, Agency for Science, Technology, and Research), Singapore . Appendix A Publisher Copyright: © 2019

PY - 2019/5

Y1 - 2019/5

N2 - Mesenchymal stem cells are the new generation of medicine for treating numerous vascular diseases and tissue defects because of their ability to secrete therapeutic factors. Poor cellular survival in an oxidative diseased tissue, however, hinders the therapeutic efficacy. To this end, we hypothesized that tethering the surface of stem cells with colloidal particles capable of discharging antioxidant cargos in response to elevated levels of hydrogen peroxide (H 2 O 2 ) would maintain survival and therapeutic activity of the stem cells. We examined this hypothesis by encapsulating epigallocatechin gallate (EGCG) and manganese oxide (MnO 2 ) nanocatalysts into particles comprising poly(D,L-lactide-co-glycolide)-block-hyaluronic acid. The MnO 2 nanocatalysts catalyzed the decomposition of H 2 O 2 into oxygen gas, which increased the internal pressure of particles and accelerated the release of EGCG by 1.5-fold. Consequently, stem cells exhibited 1.2-fold higher metabolic activity and 2.8-fold higher secretion level of pro-angiogenic factor in sub-lethal H 2 O 2 concentrations. These stem cells, in turn, performed a greater angiogenic potential with doubled number of newly formed mature blood vessels. We envisage that the results of this study will contribute to improving the therapeutic efficacy of a wide array of stem cells.

AB - Mesenchymal stem cells are the new generation of medicine for treating numerous vascular diseases and tissue defects because of their ability to secrete therapeutic factors. Poor cellular survival in an oxidative diseased tissue, however, hinders the therapeutic efficacy. To this end, we hypothesized that tethering the surface of stem cells with colloidal particles capable of discharging antioxidant cargos in response to elevated levels of hydrogen peroxide (H 2 O 2 ) would maintain survival and therapeutic activity of the stem cells. We examined this hypothesis by encapsulating epigallocatechin gallate (EGCG) and manganese oxide (MnO 2 ) nanocatalysts into particles comprising poly(D,L-lactide-co-glycolide)-block-hyaluronic acid. The MnO 2 nanocatalysts catalyzed the decomposition of H 2 O 2 into oxygen gas, which increased the internal pressure of particles and accelerated the release of EGCG by 1.5-fold. Consequently, stem cells exhibited 1.2-fold higher metabolic activity and 2.8-fold higher secretion level of pro-angiogenic factor in sub-lethal H 2 O 2 concentrations. These stem cells, in turn, performed a greater angiogenic potential with doubled number of newly formed mature blood vessels. We envisage that the results of this study will contribute to improving the therapeutic efficacy of a wide array of stem cells.

KW - Colloidal particles

KW - Epigallocatechin gallate

KW - Hydrogen peroxide

KW - Manganese oxide nanocatalysts

KW - Mesenchymal stem cells

KW - Surface tethering

UR - http://www.scopus.com/inward/record.url?scp=85061672422&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85061672422&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2019.01.039

DO - 10.1016/j.biomaterials.2019.01.039

M3 - Article

C2 - 30784768

AN - SCOPUS:85061672422

SN - 0142-9612

VL - 201

SP - 1

EP - 15

JO - Biomaterials

JF - Biomaterials

ER -