Surface plasmon resonance based immunosensing of E. coli O157:H7 in apple juice

Anand S. Subramanian, Joseph M. Irudayaraj

Research output: Contribution to journalArticlepeer-review

Abstract

Escherichia coli O157:H7 was detected in apple juice using an alkanethiol self-assembled monolayer (SAM) based surface plasmon resonance (SPR) immunosensor. Antibodies against E. coli O157:H7 were immobilized on the sensor chip, and apple juice (100%), with no added sugar, was spiked with 105, 106, 107, and 108 CFU/mL of E. coli O157:H7 and injected over the immobilized antibodies, followed by a pulse of antibodies to complete a sandwich assay. The detection limit and specificity of detection were assessed, and the deterioration of the antibody surface with time was also investigated. As low as 106 CFU/mL of E. coli O157:H7 could be detected using the sandwich assay in less than 1 h. The technique could specifically detect E. coli O157:H7 in apple juice in the presence of Staphylococcus aureus. This study shows that an SPR immunosensor has the potential for applications in rapid and label-free detection of pathogens such as E. coli O157:H7 in food.

Original languageEnglish (US)
Pages (from-to)1257-1262
Number of pages6
JournalTransactions of the ASABE
Volume49
Issue number4
StatePublished - Jul 1 2006
Externally publishedYes

Keywords

  • Apple juice
  • Biosensor
  • Escherichia coli O157:H7
  • Surface plasmon resonance

ASJC Scopus subject areas

  • Forestry
  • Food Science
  • Biomedical Engineering
  • Agronomy and Crop Science
  • Soil Science

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