Abstract
Escherichia coli O157:H7 was detected in apple juice using an alkanethiol self-assembled monolayer (SAM) based surface plasmon resonance (SPR) immunosensor. Antibodies against E. coli O157:H7 were immobilized on the sensor chip, and apple juice (100%), with no added sugar, was spiked with 105, 106, 107, and 108 CFU/mL of E. coli O157:H7 and injected over the immobilized antibodies, followed by a pulse of antibodies to complete a sandwich assay. The detection limit and specificity of detection were assessed, and the deterioration of the antibody surface with time was also investigated. As low as 106 CFU/mL of E. coli O157:H7 could be detected using the sandwich assay in less than 1 h. The technique could specifically detect E. coli O157:H7 in apple juice in the presence of Staphylococcus aureus. This study shows that an SPR immunosensor has the potential for applications in rapid and label-free detection of pathogens such as E. coli O157:H7 in food.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1257-1262 |
| Number of pages | 6 |
| Journal | Transactions of the ASABE |
| Volume | 49 |
| Issue number | 4 |
| DOIs | |
| State | Published - Jul 2006 |
| Externally published | Yes |
Keywords
- Apple juice
- Biosensor
- Escherichia coli O157:H7
- Surface plasmon resonance
ASJC Scopus subject areas
- Forestry
- Food Science
- Biomedical Engineering
- Agronomy and Crop Science
- Soil Science