Surface plasmon based substrate for enhanced fluorescence live cell imaging

Manas Ranjan Gartia, Austin Hsiao, Mayandi Sivaguru, Yi Chen, Tae Jin Kim, Yingxiao Wang, Gang Logan Liu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have created a randomly distributed nanocone substrate on silicon and coated with silver for surface-plasmonenhanced fluorescence detection and cell imaging. We observed three dimensional fluorescence enhancement on our substrate evidenced from the confocal fluorescence imaging of Chinese Hamster Ovary (CHO) cells and transfected HeLa cells grown on the substrate. The fluorescence intensity was amplified more than 100 fold as compared to glass substrate. Strong scattering within nanostructured area coupled with random total internal reflection inside the cell resulted in the observed enhancement in fluorescence further away from the substrate surface.

Original languageEnglish (US)
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1095-1097
Number of pages3
StatePublished - Dec 1 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume2

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Country/TerritoryUnited States
CitySeattle, WA
Period10/2/1110/6/11

Keywords

  • Cell imaging
  • Confocal microscopy
  • Metal enhanced fluorescence
  • Surface plasmon

ASJC Scopus subject areas

  • Control and Systems Engineering

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