Abstract

Carbon is an extremely versatile family of materials with a wide range of mechanical, optical, and mechanical properties, but many similarities in surface chemistry. As one of the most chemically stable materials known, carbon provides an outstanding platform for the development of highly tunable molecular and biomolecular interfaces. Photochemical grafting of alkenes has emerged as an attractive method for functionalizing surfaces of diamond, but many aspects of the surface chemistry and impact on biological recognition processes remain unexplored. Here we report investigations of the interaction of functionalized diamond surfaces with proteins and biological cells using X-ray photoelectron spectroscopy (XPS), atomic force microscopy, and fluorescence methods. XPS data show that functionalization of diamond with short ethylene glycol oligomers reduces the nonspecific binding of fibrinogen below the detection limit of XPS, estimated as >97% reduction over H-terminated diamond. Measurements of different forms of diamond with different roughness are used to explore the influence of roughness on nonspecific binding onto H-terminated and ethylene glycol (EG)-terminated surfaces. Finally, we use XPS to characterize the chemical stability of Escherichia coli K12 antibodies on the surfaces of diamond and amine-functionalized glass. Our results show that antibody-modified diamond surfaces exhibit increased stability in XPS and that this is accompanied by retention of biological activity in cell-capture measurements. Our results demonstrate that surface chemistry on diamond and other carbon-based materials provides an excellent platform for biomolecular interfaces with high stability and high selectivity.

Original languageEnglish (US)
Pages (from-to)983-988
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number3
DOIs
StatePublished - Jan 18 2011

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Diamond
Photoelectron Spectroscopy
Carbon
Ethylene Glycol
Biological Phenomena
Escherichia coli K12
Antibodies
Atomic Force Microscopy
Alkenes
Fibrinogen
Amines
Glass
Limit of Detection
Membrane Proteins
Fluorescence

Keywords

  • Biointerfaces
  • Cells
  • Surface chemistry

ASJC Scopus subject areas

  • General

Cite this

Surface functionalization of thin-film diamond for highly stable and selective biological interfaces. / Stavis, Courtney; Clare, Tami Lasseter; Butler, James E.; Radadia, Adarsh D.; Carr, Rogan; Zeng, Hongjun; King, William P.; Carlisle, John A.; Aksimentiev, Aleksei; Bashir, Rashid; Hamers, Robert J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, No. 3, 18.01.2011, p. 983-988.

Research output: Contribution to journalReview article

Stavis, Courtney ; Clare, Tami Lasseter ; Butler, James E. ; Radadia, Adarsh D. ; Carr, Rogan ; Zeng, Hongjun ; King, William P. ; Carlisle, John A. ; Aksimentiev, Aleksei ; Bashir, Rashid ; Hamers, Robert J. / Surface functionalization of thin-film diamond for highly stable and selective biological interfaces. In: Proceedings of the National Academy of Sciences of the United States of America. 2011 ; Vol. 108, No. 3. pp. 983-988.
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AU - Clare, Tami Lasseter

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AU - Carr, Rogan

AU - Zeng, Hongjun

AU - King, William P.

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AU - Bashir, Rashid

AU - Hamers, Robert J.

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