Surface enhanced raman spectroscopic detection of cancer biomarkers in intact cellular specimens

Dominic O. Ansari, Douglas A. Stuart, Shuming Nie

Research output: Contribution to journalConference articlepeer-review


We have developed a system for the direct spectroscopic identification of protein biomarkers in biological samples. By conjugating Raman dyes and biomolecular targeting agents to gold nanoparticles, we have produced highly selective optically encoded probes. The nanostructures are grown within a cellular sample to generate a surface that is highly active for surface enhanced Raman spectroscopy (SERS). Both in vitro characterizations of SERS detection as well as the sensitive and specific detection of cancer biomarkers in cultured cancer cells have been demonstrated.

Original languageEnglish (US)
Article number14
Pages (from-to)82-90
Number of pages9
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
StatePublished - Jul 21 2005
Externally publishedYes
EventImaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III - San Jose, CA, United States
Duration: Jan 24 2005Jan 27 2005


  • Biomarkers
  • Cancer
  • Diagnosis
  • Immunoassay
  • Nanoarticles
  • Nanotechnology
  • Optical encoding
  • SERS
  • Surface enhanced Raman scattering

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


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