Abstract
We have developed a system for the direct spectroscopic identification of protein biomarkers in biological samples. By conjugating Raman dyes and biomolecular targeting agents to gold nanoparticles, we have produced highly selective optically encoded probes. The nanostructures are grown within a cellular sample to generate a surface that is highly active for surface enhanced Raman spectroscopy (SERS). Both in vitro characterizations of SERS detection as well as the sensitive and specific detection of cancer biomarkers in cultured cancer cells have been demonstrated.
| Original language | English (US) |
|---|---|
| Article number | 14 |
| Pages (from-to) | 82-90 |
| Number of pages | 9 |
| Journal | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
| Volume | 5699 |
| DOIs | |
| State | Published - 2005 |
| Externally published | Yes |
| Event | Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III - San Jose, CA, United States Duration: Jan 24 2005 → Jan 27 2005 |
Keywords
- Biomarkers
- Cancer
- Diagnosis
- Immunoassay
- Nanoarticles
- Nanotechnology
- Optical encoding
- SERS
- Surface enhanced Raman scattering
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Radiology Nuclear Medicine and imaging
- Biomaterials