Surface-enhanced Raman spectra of eye lens pigments

Shuming Nie, Carolina G. Castillo, Katrina L. Bergbauer, John F.R. Kuck, Igor R. Nabiev, Nai Teng Yu

Research output: Contribution to journalArticlepeer-review

Abstract

Surface-enhanced Raman spectroscopy (SERS) has been applied to study lenticular pigments that are present in the eyes of certain diuranally active animals. Using Ag hydrosols pre-aggregated with NaClO4, we have obtained SERS spectra from dilute solutions of various model pigment compounds, including kynurenine, N-formylkynrenine, 3-hydroxykyn-urenine, β-carboline, bityrosine, anthranilic acid, 3-hydroxyanthranilic acid, and oxindole. The results obtained from these model compounds show that SERS is a particularly sensitive technique for the identification of lens pigments. We also report a procedure that enables high-quality SERS data to be obtained for the yellow pigments in the lens homogenates of grey squirrels, ground squirrels, and chipmunks.

Original languageEnglish (US)
Pages (from-to)571-575
Number of pages5
JournalApplied Spectroscopy
Volume44
Issue number4
DOIs
StatePublished - 1990
Externally publishedYes

ASJC Scopus subject areas

  • Instrumentation
  • Spectroscopy

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